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-Structure paper
タイトル | Allosteric transcription stimulation by RNA polymerase II super elongation complex. |
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ジャーナル・号・ページ | Mol Cell, Vol. 81, Issue 16, Page 3386-3399.e10, Year 2021 |
掲載日 | 2021年8月19日 |
![]() | Ying Chen / Seychelle M Vos / Christian Dienemann / Momchil Ninov / Henning Urlaub / Patrick Cramer / ![]() |
PubMed 要旨 | The super elongation complex (SEC) contains the positive transcription elongation factor b (P-TEFb) and the subcomplex ELL2-EAF1, which stimulates RNA polymerase II (RNA Pol II) elongation. Here, we ...The super elongation complex (SEC) contains the positive transcription elongation factor b (P-TEFb) and the subcomplex ELL2-EAF1, which stimulates RNA polymerase II (RNA Pol II) elongation. Here, we report the cryoelectron microscopy (cryo-EM) structure of ELL2-EAF1 bound to a RNA Pol II elongation complex at 2.8 Å resolution. The ELL2-EAF1 dimerization module directly binds the RNA Pol II lobe domain, explaining how SEC delivers P-TEFb to RNA Pol II. The same site on the lobe also binds the initiation factor TFIIF, consistent with SEC binding only after the transition from transcription initiation to elongation. Structure-guided functional analysis shows that the stimulation of RNA elongation requires the dimerization module and the ELL2 linker that tethers the module to the RNA Pol II protrusion. Our results show that SEC stimulates elongation allosterically and indicate that this stimulation involves stabilization of a closed conformation of the RNA Pol II active center cleft. |
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手法 | EM (単粒子) |
解像度 | 2.8 - 4.14 Å |
構造データ | ![]() EMDB-12966: ![]() EMDB-12967: ![]() EMDB-12968: EMDB-12969: Structure of active transcription elongation complex Pol II-DSIF (SPT5-KOW5)-ELL2-EAF1, Composite map 1 (Primary map) ![]() EMDB-12970: ![]() EMDB-12971: ![]() EMDB-12972: EMDB-12973: Structure of active transcription elongation complex Pol II-DSIF-ELL2-EAF1, composite map 2 EMDB-12974: Structure of active transcription elongation complex Pol II-DSIF (SPT5-KOW5) alone, Map 11, Global map |
化合物 | ![]() ChemComp-MG: ![]() ChemComp-ZN: |
由来 |
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![]() | TRANSCRIPTION / Pol II / Super elongation complex / ELL2 / EAF1 |