EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure and assembly of the mammalian mitochondrial supercomplex CIIICIV.
ジャーナル・号・ページ	Nature, Vol. 598, Issue 7880, Page 364-367, Year 2021
掲載日	2021年10月6日
著者	Irene Vercellino / Leonid A Sazanov /
PubMed 要旨	The enzymes of the mitochondrial electron transport chain are key players of cell metabolism. Despite being active when isolated, in vivo they associate into supercomplexes, whose precise role is ...The enzymes of the mitochondrial electron transport chain are key players of cell metabolism. Despite being active when isolated, in vivo they associate into supercomplexes, whose precise role is debated. Supercomplexes CIIICIV (refs. ), CICIII (ref. ) and CICIIICIV (respirasome) exist in mammals, but in contrast to CICIII and the respirasome, to date the only known eukaryotic structures of CIIICIV come from Saccharomyces cerevisiae and plants, which have different organization. Here we present the first, to our knowledge, structures of mammalian (mouse and ovine) CIIICIV and its assembly intermediates, in different conformations. We describe the assembly of CIIICIV from the CIII precursor to the final CIIICIV conformation, driven by the insertion of the N terminus of the assembly factor SCAF1 (ref. ) deep into CIII, while its C terminus is integrated into CIV. Our structures (which include CICIII and the respirasome) also confirm that SCAF1 is exclusively required for the assembly of CIIICIV and has no role in the assembly of the respirasome. We show that CIII is asymmetric due to the presence of only one copy of subunit 9, which straddles both monomers and prevents the attachment of a second copy of SCAF1 to CIII, explaining the presence of one copy of CIV in CIIICIV in mammals. Finally, we show that CIII and CIV gain catalytic advantage when assembled into the supercomplex and propose a role for CIIICIV in fine tuning the efficiency of electron transfer in the electron transport chain.
リンク	Nature / PubMed:34616041
手法	EM (単粒子)
解像度	2.6 - 3.6 Å
構造データ	12702 7o37 EMDB-12702, PDB-7o37: Murine supercomplex CIII2CIV in the assembled locked conformation 手法: EM (単粒子) / 解像度: 3.2 Å 12703 7o3c EMDB-12703, PDB-7o3c: Murine supercomplex CIII2CIV in the mature unlocked conformation 手法: EM (単粒子) / 解像度: 3.3 Å 12705 7o3e EMDB-12705, PDB-7o3e: Murine supercomplex CIII2CIV in the intermediate locked conformation 手法: EM (単粒子) / 解像度: 3.6 Å 12706 7o3h EMDB-12706, PDB-7o3h: Murine CIII2 focus-refined from supercomplex CICIII2 手法: EM (単粒子) / 解像度: 2.6 Å
化合物	ChemComp-3PE: 1,2-Distearoyl-sn-glycerophosphoethanolamine / DSPE / リン脂質YM ChemComp-CDL: CARDIOLIPIN / リン脂質YM ChemComp-HEM: PROTOPORPHYRIN IX CONTAINING FE ChemComp-HEC: HEME C ChemComp-FES: FE2/S2 (INORGANIC) CLUSTER ChemComp-PC1: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / リン脂質YM ChemComp-TGL: TRISTEAROYLGLYCEROL / トリステアリン ChemComp-CU: COPPER (II) ION ChemComp-NA: Unknown entry / ナトリウムカチオン ChemComp-HEA: HEME-A ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-CUA: DINUCLEAR COPPER ION ChemComp-ZN*: Unknown entry
由来	mus musculus (ハツカネズミ) Mouse (ネズミ)
キーワード	MEMBRANE PROTEIN / mitochondria / respiratory chain / supercomplex / OXIDOREDUCTASE / complex III / complex IV