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-Structure paper
タイトル | Structural basis of nucleosome transcription mediated by Chd1 and FACT. |
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ジャーナル・号・ページ | Nat Struct Mol Biol, Vol. 28, Issue 4, Page 382-387, Year 2021 |
掲載日 | 2021年4月12日 |
![]() | Lucas Farnung / Moritz Ochmann / Maik Engeholm / Patrick Cramer / ![]() |
PubMed 要旨 | Efficient transcription of RNA polymerase II (Pol II) through nucleosomes requires the help of various factors. Here we show biochemically that Pol II transcription through a nucleosome is ...Efficient transcription of RNA polymerase II (Pol II) through nucleosomes requires the help of various factors. Here we show biochemically that Pol II transcription through a nucleosome is facilitated by the chromatin remodeler Chd1 and the histone chaperone FACT when the elongation factors Spt4/5 and TFIIS are present. We report cryo-EM structures of transcribing Saccharomyces cerevisiae Pol II-Spt4/5-nucleosome complexes with bound Chd1 or FACT. In the first structure, Pol II transcription exposes the proximal histone H2A-H2B dimer that is bound by Spt5. Pol II has also released the inhibitory DNA-binding region of Chd1 that is poised to pump DNA toward Pol II. In the second structure, Pol II has generated a partially unraveled nucleosome that binds FACT, which excludes Chd1 and Spt5. These results suggest that Pol II progression through a nucleosome activates Chd1, enables FACT binding and eventually triggers transfer of FACT together with histones to upstream DNA. |
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手法 | EM (単粒子) |
解像度 | 2.7 - 3.5 Å |
構造データ | EMDB-12449, PDB-7nkx: EMDB-12450, PDB-7nky: ![]() EMDB-12666: ![]() EMDB-12667: ![]() EMDB-12668: ![]() EMDB-12669: ![]() EMDB-12670: ![]() EMDB-12671: |
化合物 | ![]() ChemComp-ZN: ![]() ChemComp-MG: ![]() ChemComp-ADP: ![]() ChemComp-BEF: |
由来 |
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![]() | TRANSCRIPTION / chromatin remodelling / nucleosome / chromatin / elongation / histone chaperone |