EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Catalytic cycling of human mitochondrial Lon protease.
ジャーナル・号・ページ	Structure, Vol. 30, Issue 9, Page 1254-11268.e7, Year 2022
掲載日	2022年9月1日
著者	Inayathulla Mohammed / Kai A Schmitz / Niko Schenck / Dimitrios Balasopoulos / Annika Topitsch / Timm Maier / Jan Pieter Abrahams /
PubMed 要旨	The mitochondrial Lon protease (LonP1) regulates mitochondrial health by removing redundant proteins from the mitochondrial matrix. We determined LonP1 in eight nucleotide-dependent conformational ...The mitochondrial Lon protease (LonP1) regulates mitochondrial health by removing redundant proteins from the mitochondrial matrix. We determined LonP1 in eight nucleotide-dependent conformational states by cryoelectron microscopy (cryo-EM). The flexible assembly of N-terminal domains had 3-fold symmetry, and its orientation depended on the conformational state. We show that a conserved structural motif around T803 with a high similarity to the trypsin catalytic triad is essential for proteolysis. We show that LonP1 is not regulated by redox potential, despite the presence of two conserved cysteines at disulfide-bonding distance in its unfoldase core. Our data indicate how sequential ATP hydrolysis controls substrate protein translocation in a 6-fold binding change mechanism. Substrate protein translocation, rather than ATP hydrolysis, is a rate-limiting step, suggesting that LonP1 is a Brownian ratchet with ATP hydrolysis preventing translocation reversal. 3-fold rocking motions of the flexible N-domain assembly may assist thermal unfolding of the substrate protein.
リンク	Structure / PubMed:35870450
手法	EM (単粒子)
解像度	3.8 - 15.0 Å
構造データ	12306 7nfy EMDB-12306, PDB-7nfy: P1a-state of wild type human mitochondrial LONP1 protease with bound substrate protein and ATPgS 手法: EM (単粒子) / 解像度: 3.9 Å 12307 7ng4 EMDB-12307, PDB-7ng4: P1b-state of wild type human mitochondrial LONP1 protease with bound endogenous substrate protein and in presence of ATP/ADP mix 手法: EM (単粒子) / 解像度: 4.4 Å 12308 7ng5 EMDB-12308, PDB-7ng5: P1c-state of wild type human mitochondrial LONP1 protease with bound substrate protein in presence of ATP/ADP mix 手法: EM (単粒子) / 解像度: 3.8 Å 12312 7ngc EMDB-12312, PDB-7ngc: P2a-state of wild type human mitochondrial LONP1 protease with bound substrate protein and in presence of ATPgS 手法: EM (単粒子) / 解像度: 7.5 Å 12313 7ngf EMDB-12313, PDB-7ngf: P2c-state of wild type human mitochondrial LONP1 protease with bound endogenous substrate protein and in presence of ATP/ADP mix 手法: EM (単粒子) / 解像度: 5.6 Å 12315 7ngl EMDB-12315, PDB-7ngl: R-state of wild type human mitochondrial LONP1 protease bound to endogenous ADP 手法: EM (単粒子) / 解像度: 3.8 Å 12316 7ngp EMDB-12316, PDB-7ngp: D1-state of wild type human mitochondrial LONP1 protease 手法: EM (単粒子) / 解像度: 15.0 Å 12317 7ngq EMDB-12317, PDB-7ngq: Human mitochondrial Lon protease homolog, D2-state 手法: EM (単粒子) / 解像度: 12.0 Å 13102 7oxo EMDB-13102, PDB-7oxo: human LonP1, R-state, incubated in AMPPCP 手法: EM (単粒子) / 解像度: 3.9 Å
化合物	ChemComp-AGS: PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / ATP-γ-S / ATP-gamma-S, エネルギー貯蔵分子類似体YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子*YM
由来	homo sapiens (ヒト)
キーワード	MOTOR PROTEIN / human mitochondrial AAA+ protease / protease / unfolds / hexamer / AAA+ protein / chaperone