EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	A conserved rRNA switch is central to decoding site maturation on the small ribosomal subunit.
ジャーナル・号・ページ	Sci Adv, Vol. 7, Issue 23, Year 2021
掲載日	2021年6月4日
著者	Andreas Schedlbauer / Idoia Iturrioz / Borja Ochoa-Lizarralde / Tammo Diercks / Jorge Pedro López-Alonso / José Luis Lavin / Tatsuya Kaminishi / Retina Çapuni / Neha Dhimole / Elisa de Astigarraga / David Gil-Carton / Paola Fucini / Sean R Connell /
PubMed 要旨	While a structural description of the molecular mechanisms guiding ribosome assembly in eukaryotic systems is emerging, bacteria use an unrelated core set of assembly factors for which high- ...While a structural description of the molecular mechanisms guiding ribosome assembly in eukaryotic systems is emerging, bacteria use an unrelated core set of assembly factors for which high-resolution structural information is still missing. To address this, we used single-particle cryo-electron microscopy to visualize the effects of bacterial ribosome assembly factors RimP, RbfA, RsmA, and RsgA on the conformational landscape of the 30 ribosomal subunit and obtained eight snapshots representing late steps in the folding of the decoding center. Analysis of these structures identifies a conserved secondary structure switch in the 16 ribosomal RNA central to decoding site maturation and suggests both a sequential order of action and molecular mechanisms for the assembly factors in coordinating and controlling this switch. Structural and mechanistic parallels between bacterial and eukaryotic systems indicate common folding features inherent to all ribosomes.
リンク	Sci Adv / PubMed:34088665 / PubMed Central
手法	EM (単粒子) / NMR (溶液)
解像度	2.75 - 4.9 Å
構造データ	11751 7af3 EMDB-11751, PDB-7af3: Bacterial 30S ribosomal subunit assembly complex state M (head domain) 手法: EM (単粒子) / 解像度: 2.82 Å 11753 7af5 EMDB-11753, PDB-7af5: Bacterial 30S ribosomal subunit assembly complex state I (head domain) 手法: EM (単粒子) / 解像度: 2.96 Å 11756 7af8 EMDB-11756, PDB-7af8: Bacterial 30S ribosomal subunit assembly complex state E (head domain) 手法: EM (単粒子) / 解像度: 2.75 Å 11758 7afa EMDB-11758, PDB-7afa: Bacterial 30S ribosomal subunit assembly complex state F (head domain) 手法: EM (単粒子) / 解像度: 2.95 Å 11761 7afd EMDB-11761, PDB-7afd: Bacterial 30S ribosomal subunit assembly complex state A (head domain) 手法: EM (単粒子) / 解像度: 3.44 Å 11765 7afh EMDB-11765, PDB-7afh: Bacterial 30S ribosomal subunit assembly complex state C (head domain) 手法: EM (単粒子) / 解像度: 3.59 Å 11766 7afi EMDB-11766, PDB-7afi: Bacterial 30S ribosomal subunit assembly complex state C (body domain) 手法: EM (単粒子) / 解像度: 3.53 Å 11768 7afk EMDB-11768, PDB-7afk: Bacterial 30S ribosomal subunit assembly complex state D (head domain) 手法: EM (単粒子) / 解像度: 4.9 Å 11769 7afl EMDB-11769, PDB-7afl: Bacterial 30S ribosomal subunit assembly complex state D (multibody refinement for body domain of 30S ribosome) 手法: EM (単粒子) / 解像度: 4.2 Å 11771 7afn EMDB-11771, PDB-7afn: Bacterial 30S ribosomal subunit assembly complex state B (head domain) 手法: EM (単粒子) / 解像度: 3.86 Å 11772 7afo EMDB-11772, PDB-7afo: Bacterial 30S ribosomal subunit assembly complex state B (body domain) 手法: EM (単粒子) / 解像度: 3.93 Å 12239 7bod EMDB-12239, PDB-7bod: Bacterial 30S ribosomal subunit assembly complex state M (body domain) 手法: EM (単粒子) / 解像度: 2.88 Å 12240 7boe EMDB-12240, PDB-7boe: Bacterial 30S ribosomal subunit assembly complex state M (Consensus refinement) 手法: EM (単粒子) / 解像度: 2.9 Å 12241 7bof EMDB-12241, PDB-7bof: Bacterial 30S ribosomal subunit assembly complex state I (body domain) 手法: EM (単粒子) / 解像度: 2.92 Å 12242 7bog EMDB-12242, PDB-7bog: Bacterial 30S ribosomal subunit assembly complex state E (body domain) 手法: EM (単粒子) / 解像度: 2.75 Å 12243 7boh EMDB-12243, PDB-7boh: Complete Bacterial 30S ribosomal subunit assembly complex state E (+RbfA)(Consensus Refinement) 手法: EM (単粒子) / 解像度: 2.82 Å 12244 7boi EMDB-12244, PDB-7boi: Bacterial 30S ribosomal subunit assembly complex state F (multibody refinement for body domain of 30S ribosome) 手法: EM (単粒子) / 解像度: 2.98 Å 12245 7nar EMDB-12245, PDB-7nar: Complete Bacterial 30S ribosomal subunit assembly complex state F (+RsgA)(Consensus Refinement) 手法: EM (単粒子) / 解像度: 3.0 Å 12246 7nas EMDB-12246, PDB-7nas: Bacterial 30S ribosomal subunit assembly complex state A (multibody refinement for body domain of 30S ribosome) 手法: EM (単粒子) / 解像度: 3.31 Å 12247 7nat EMDB-12247, PDB-7nat: Bacterial 30S ribosomal subunit assembly complex state A (Consensus refinement) 手法: EM (単粒子) / 解像度: 3.59 Å 12248 7nau EMDB-12248, PDB-7nau: Bacterial 30S ribosomal subunit assembly complex state C (Consensus Refinement) 手法: EM (単粒子) / 解像度: 3.78 Å 12249 7nav EMDB-12249, PDB-7nav: Bacterial 30S ribosomal subunit assembly complex state D (Consensus refinement) 手法: EM (単粒子) / 解像度: 4.8 Å 12251 7nax EMDB-12251, PDB-7nax: Complete Bacterial 30S ribosomal subunit assembly complex state I (Consensus Refinement) 手法: EM (単粒子) / 解像度: 2.96 Å PDB-7afq: Ribosome binding factor A (RbfA) 手法: SOLUTION NMR PDB-7afr: Ribosome maturation factor RimP (apo) 手法: SOLUTION NMR
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ZN: Unknown entry ChemComp-GNP: PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER / Gpp(NH)p / GppNHp, GMPPNP, エネルギー貯蔵分子類似体*YM
由来	escherichia coli (大腸菌) escherichia coli (strain k12) (大腸菌) escherichia coli k-12 (大腸菌) escherichia coli o26:h11 (大腸菌)
キーワード	RIBOSOME / Cryo-EM / 30S biogenesis / ribosome assembly / RbfA / RsgA / YjeQ / RimP / KsgA / RsmA / RNA BINDING PROTEIN / Solution state NMR / RbfA. / RimP.