+Open data
-Basic information
Entry | Database: PDB / ID: 7afr | |||||||||
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Title | Ribosome maturation factor RimP (apo) | |||||||||
Components | Ribosome maturation factor RimP | |||||||||
Keywords | RNA BINDING PROTEIN / solution state NMR / 30S biogenesis / ribosome assembly / RimP. | |||||||||
Function / homology | Function and homology information | |||||||||
Biological species | Escherichia coli (E. coli) | |||||||||
Method | SOLUTION NMR / simulated annealing / molecular dynamics | |||||||||
Authors | Schedlbauer, A. / Iturrioz, I. / Ochoa-Lizarralde, B. / Diercks, T. / Fucini, P. / Connell, S. | |||||||||
Funding support | Spain, 2items
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Citation | Journal: Sci Adv / Year: 2021 Title: A conserved rRNA switch is central to decoding site maturation on the small ribosomal subunit. Authors: Andreas Schedlbauer / Idoia Iturrioz / Borja Ochoa-Lizarralde / Tammo Diercks / Jorge Pedro López-Alonso / José Luis Lavin / Tatsuya Kaminishi / Retina Çapuni / Neha Dhimole / Elisa de ...Authors: Andreas Schedlbauer / Idoia Iturrioz / Borja Ochoa-Lizarralde / Tammo Diercks / Jorge Pedro López-Alonso / José Luis Lavin / Tatsuya Kaminishi / Retina Çapuni / Neha Dhimole / Elisa de Astigarraga / David Gil-Carton / Paola Fucini / Sean R Connell / Abstract: While a structural description of the molecular mechanisms guiding ribosome assembly in eukaryotic systems is emerging, bacteria use an unrelated core set of assembly factors for which high- ...While a structural description of the molecular mechanisms guiding ribosome assembly in eukaryotic systems is emerging, bacteria use an unrelated core set of assembly factors for which high-resolution structural information is still missing. To address this, we used single-particle cryo-electron microscopy to visualize the effects of bacterial ribosome assembly factors RimP, RbfA, RsmA, and RsgA on the conformational landscape of the 30 ribosomal subunit and obtained eight snapshots representing late steps in the folding of the decoding center. Analysis of these structures identifies a conserved secondary structure switch in the 16 ribosomal RNA central to decoding site maturation and suggests both a sequential order of action and molecular mechanisms for the assembly factors in coordinating and controlling this switch. Structural and mechanistic parallels between bacterial and eukaryotic systems indicate common folding features inherent to all ribosomes. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 7afr.cif.gz | 901.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb7afr.ent.gz | 772.3 KB | Display | PDB format |
PDBx/mmJSON format | 7afr.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/af/7afr ftp://data.pdbj.org/pub/pdb/validation_reports/af/7afr | HTTPS FTP |
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-Related structure data
Related structure data | 7af3C 7af5C 7af8C 7afaC 7afdC 7afhC 7afiC 7afkC 7aflC 7afnC 7afoC 7afqC 7bodC 7boeC 7bofC 7bogC 7bohC 7boiC 7narC 7nasC 7natC 7nauC 7navC 7naxC C: citing same article (ref.) |
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Similar structure data | |
Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 16737.992 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) Gene: rimP, yhbC, A6581_13520, A6592_14330, A6V01_00515, A8C65_02275, A9819_18200, A9P13_03050, A9R57_12705, A9X72_02945, AC067_23780, AC789_1c35400, ACN002_3263, ACU57_14550, ACU90_11670, AJ318_ ...Gene: rimP, yhbC, A6581_13520, A6592_14330, A6V01_00515, A8C65_02275, A9819_18200, A9P13_03050, A9R57_12705, A9X72_02945, AC067_23780, AC789_1c35400, ACN002_3263, ACU57_14550, ACU90_11670, AJ318_05135, AM270_15615, AM446_04425, AM464_08475, AM465_07740, AMK83_06445, AML07_19235, AML35_13990, APT94_23095, APU18_16510, APZ14_04190, ARC77_12300, AU473_05840, AUQ13_02970, AUS26_18365, AW059_15280, AW106_13165, AWB10_02960, AWE53_007750, AWF59_011795, AWG78_015830, AWG90_020515, AZZ83_001444, B6V57_17915, B7C53_06465, B9M99_10360, B9N33_16975, B9T59_16360, BANRA_01790, BANRA_03978, BANRA_04186, BB545_09885, BEN53_19595, BFD68_02460, BHF03_08615, BHF46_07210, BHS81_19095, BHS87_17860, BIQ87_17930, BIU72_05300, BIZ41_14495, BJJ90_02875, BK248_21925, BK292_10325, BK296_12945, BK334_09665, BK373_10280, BK375_17180, BK383_15860, BMA87_01190, BMT49_25700, BMT91_09135, BvCms12BK_01026, BvCms2454_04843, BvCms28BK_03353, BvCms35BK_01522, BvCmsC61A_01482, BvCmsHHP019_03132, BvCmsHHP056_02505, BvCmsKKP036_00265, BvCmsKKP061_00867, BvCmsKSNP073_02141, BvCmsKSNP081_00265, BvCmsKSNP120_03551, BvCmsKSP011_02071, BvCmsKSP024_00304, BvCmsKSP026_00481, BvCmsKSP045_01629, BvCmsKSP058_01861, BvCmsKSP067_01443, BvCmsKSP076_01609, BvCmsNSNP036_02130, BvCmsNSP006_04619, BvCmsNSP007_02230, BvCmsNSP047_04139, BvCmsNSP072_03166, BvCmsOUP014_03611, BvCmsSINP011_04479, BvCmsSINP022_03542, BvCmsSIP019_00386, BvCmsSIP044_00116, BWI89_22175, C2M16_04320, C2U48_17655, C4K41_03675, C4M78_03215, C5715_22690, C5N07_18175, C5P01_18940, C5P44_00535, C6669_12125, C6B13_18120, C7235_03330, C7B02_15770, C7B06_16695, C7B07_10705, C7B08_03460, C7B18_13725, C9098_07895, C9114_15350, C9141_00495, C9160_02445, C9162_04215, C9201_13770, C9306_00520, C9E25_11375, C9E67_03480, C9Z03_07385, C9Z23_02310, C9Z28_00490, C9Z29_07085, C9Z37_14765, C9Z39_14380, C9Z43_00485, C9Z69_11070, C9Z70_06870, C9Z78_03420, C9Z89_09170, CA593_10450, CCZ14_12150, CCZ17_15250, CDC27_16980, CDL37_12105, CF006_16775, CG692_07190, CI641_009150, CI693_03565, CI694_20945, CIG45_16155, CJU63_19030, CJU64_18910, CMR93_08945, CO706_14525, COD30_12805, COD46_10650, CQB02_14455, CQP61_03755, CR538_03180, CR539_21140, CRD98_04820, CRE06_06720, CRJUMX01_260087, CRM83_23605, CRT43_19210, CRX46_03850, CSB64_06490, CT146_02950, CUB99_16240, CVH05_10885, CWM24_22975, CWS33_08270, D0X26_08985, D1912_12210, D2184_10400, D2185_00550, D2188_02685, D3821_08430, D3822_14680, D3M98_06670, D3O91_07730, D3P01_09055, D3P02_05570, D3Y67_18395, D4011_13620, D4074_02330, D4628_02550, D4636_02785, D4638_00685, D4660_02725, D4718_08975, D4L91_11845, D4M06_11120, D4U49_10465, D4U85_14225, D4V08_01260, D5H35_09675, D5I97_10565, D6004_08020, D6C36_14055, D6D43_15405, D6T60_15285, D6T98_02305, D6W00_13910, D6X36_05255, D6X63_01325, D6X76_04230, D7K33_12750, D7K63_12115, D7K66_04390, D7W70_15750, D7Y10_03485, D7Z75_02840, D8Y65_13370, D9610_07705, D9C99_05015, D9D31_09850, D9D33_00515, D9D43_03510, D9D44_04305, D9D94_03385, D9E13_09760, D9E19_10295, D9E34_10730, D9E49_09490, D9E73_12375, D9F17_07095, D9F32_01135, D9G11_02975, D9G29_06920, D9G42_16795, D9G48_22395, D9G69_00995, D9G95_16980, D9H10_06565, D9H36_12500, D9H53_01090, D9H68_07455, D9H70_01525, D9H94_03415, D9I18_07070, D9I20_02730, D9I37_14605, D9I87_04955, D9I88_16545, D9I97_11395, D9J11_01025, D9J44_07745, D9J46_20545, D9J52_07810, D9J58_06005, D9J60_11080, D9J63_16805, D9J78_05025, D9K02_05975, D9K48_12150, D9K54_17935, D9L89_05265, D9L99_02555, D9S45_00150, D9X77_02225, D9X97_03015, D9Z28_03265, DAH18_20505, DAH26_17210, DAH30_12980, DAH32_14080, DAH34_01875, DAH37_04360, DAH43_16675, DB359_10675, DBQ99_04145, DD762_19135, DEN86_08165, DEN89_09300, DEN97_16030, DEO04_19500, DEO19_06325, DEO20_07555, DIV22_03080, DJ487_12870, DJ492_21815, DJ503_10870, DK132_15395, DL251_15040, DL257_05545, DL292_14605, DL326_05955, DL455_12595, DL479_06485, DL530_21135, DL545_03765, DL705_01195, DL800_23160, DL979_03350, DLT82_02550, DLU50_08445, DLU67_08270, DLU82_10225, DLW60_07385, DLW88_04690, DLX38_07775, DLY41_08050, DLY44_02205, DM102_15060, DM129_03900, DM155_07540, DM267_13960, DM272_02325, DM280_08950, DM296_04730, DM382_07255, DM820_04520, DM962_02535, DM973_04545, DMC44_07025, DMI04_04165, DMI53_12610, DMO02_15300, DMY83_09815, DMZ50_12315, DN660_01225, DN700_04490, DN703_00975, DN808_01785, DNB37_13600, DNC98_07055, DND16_06430, DND79_01225, DNI21_14825, DNJ62_11965, DNK12_02730, DNQ45_19755, DNR35_05290, DNR41_11080, DNW42_05300, DNX19_05475, DNX30_11260, DOE35_12505, DOM23_01535, DOS18_12925, DOT81_04150, DOU81_12270, DOY22_13980, DOY56_12140, DOY61_04590, DOY67_01180, DP265_06450, DP277_10000, DQE91_01160, DQF36_20850, DQF57_03510, DQF71_07055, DQF72_06355, DQG35_02725, DQO13_01190, DQP61_01665, DRP48_13805, DRW19_01190, DS143_06665, DS721_10985, DS732_23590, DS966_19175, DT034_01165, DTL43_04190, DTM10_07090, DTM45_14405, DTZ20_11985, DU309_11240, DU321_08565, DU333_16815, DVB38_07290, DW236_11980, DWB25_03315, DXT69_05625, DXT71_00545, DXT73_13265, DXX80_009485, E0I42_14235, E0K84_20585, E0L04_19770, E0L12_03810, E2112_05640, E2114_08285, E2115_05735, E2119_05765, E2127_02470, E2128_02600, E2129_05655, E2134_15700, E2135_07975, E2148_12390, E2855_04121, E2863_03946, E4K55_06555, E4K60_03320, E4K61_09175, E5P22_12890, E5P28_13015, E5P37_13240, E5S42_11910, E5S46_04245, E5S47_02865, E5S56_15530, E5S58_02830, E5S61_05810, EA159_13410, EA167_07035, EA189_10710, EA191_13025, EA198_19520, EA200_07260, EA203_14945, EA213_02390, EA214_08705, EA218_15110, EA222_06430, EA225_06975, EA231_14825, EA232_14625, EA233_06070, EA242_07640, EA245_09665, EA250_06530, EA410_15875, EA429_03180, EA434_19670, EA435_15260, EA834_14920, EAI42_15040, EAI46_15085, EAI52_09995, EAM59_00915, EAN70_10640, EAN77_10380, EAX79_13125, EB476_09145, EB509_07095, EB510_12300, EB515_06920, EBA46_01560, EBA84_05725, EBJ06_06030, EBM08_05350, EC3234A_53c00490, EC3426_04319, EC382_14550, EC95NR1_02552, ECONIH1_18750, ECTO124_00619, ECTO6_00586, ED225_04165, ED307_11680, ED600_09295, ED607_10320, ED611_04175, ED648_02810, ED903_04550, ED944_07155, EEA45_06835, EEP23_06195, EF082_03885, EF173_16900, EG075_10005, EG599_06060, EG796_01085, EG808_04740, EGC26_04810, EGT48_12345, EGU87_04110, EH186_10365, EH412_00890, EHD45_09310, EHD63_05170, EHD79_19375, EHH55_24255, EHJ36_00890, EHJ66_05985, EHV81_00450, EHV90_06065, EHW09_05495, EHX09_11690, EI021_03960, EI028_15155, EI032_08700, EI041_02105, EIA08_12605, EIA21_02775, EIZ93_15760, EJ366_26100, EJC75_15120, EKI52_18340, ELT17_03400, ELT20_05150, ELT22_04740, ELT23_06870, ELT33_08790, ELT48_07015, ELT49_04545, ELT58_00540, ELU82_09410, ELU85_07030, ELU96_15070, ELV05_02170, ELV08_03090, ELV13_06450, ELV15_01725, ELV22_10740, ELV24_06460, ELV28_06240, ELX56_11845, ELX70_11310, ELX76_04020, ELX79_02845, ELX83_01980, ELY05_03155, ELY23_12930, ELY24_06190, ELY41_02060, ELY48_01080, ELY50_10975, EO241_09475, EPS76_01470, EPS91_00535, EPS94_08350, EPT01_00520, EPU41_18025, EQ823_04945, EQ825_16430, EQ830_04625, ERL57_13330, ERS085365_00561, ERS085366_00575, ERS085374_01907, ERS085379_00394, ERS085383_00718, ERS085386_00509, ERS085404_02208, ERS085406_02910, ERS085416_00333, ERS139211_00574, ERS150873_04325, ERS150876_01006, EST51_02850, EVY14_07620, EWK56_02880, EXM29_19890, ExPECSC019_00733, ExPECSC038_02681, ExPECSC065_00152, EXX06_09585, EXX13_07220, EXX23_07435, EXX24_06255, EXX53_05215, EXX55_08940, EXX71_13295, EXX78_16835, EXX87_04485, EYD11_02750, EYX82_02350, EYX99_26670, EYY27_17450, EYY34_08680, EYY78_15515, F1E13_04755, F1E19_22160, F7F00_12275, F7F11_09140, F7F18_01100, F7F23_11550, F7F26_00510, F7F29_05220, F7G01_14085, F7G03_16255, F9059_01215, F9Z74_11735, FAF34_018990, FE846_13840, FKO60_06135, FNJ67_03680, FNJ69_14760, FNJ83_20460, FQ022_08085, FQ915_16575, FQR64_03140, FQU83_04615, FQZ46_16235, FRV13_20650, FV293_08480, FV295_10420, FV438_11005, FVB16_12900, FWK02_30950, FY127_14860, FZ043_21930, GFU40_08290, GFU45_16445, GFU47_23645, GHR40_07700, GII67_12855, GII91_02620, GIY13_09410, GIY19_08440, GJ11_20705, GJ638_05280, GJD97_02990, GKE15_04560, GKE22_12100, GKE24_04550, GKE26_04235, GKE29_02570, GKE31_04515, GKE39_10640, GKE46_11105, GKE58_04470, GKE60_04205, GKE64_10430, GKE77_04900, GKE87_00805, GKE92_01045, GKE93_06080, GKF00_04240, GKF03_05185, GKF28_11940, GKF34_04535, GKF47_04530, GKF74_09325, GKF86_04155, GKF89_14480, GKG08_09990, GKG09_10130, GKG11_10335, GKG12_08430, GKG22_10250, GKG29_10290, GN312_06535, GNZ00_04975, GNZ02_01185, GNZ03_06215, GP654_17605, GP661_13150, GP664_11975, GP666_12855, GP689_13980, GP700_08285, GP712_11280, GP720_14900, GP727_07890, GP912_13015, GP935_13235, GP946_08565, GP950_18120, GQA06_13100, GQE22_11445, GQE30_02675, GQE34_11815, GQE42_06055, GQE47_10010, GQE51_04775, GQE58_09360, GQE64_09830, GQE68_06305, GQE88_18540, GQE93_14745, GQL64_21185, GQM06_01810, GQM09_10960, GQM13_09315, GQM17_12990, GQN16_09395, GQN33_10425, GQS26_10800, GRW42_15825, GRW80_12150, HmCms169_01197, HmCms184_00230, HmCmsJML074_04857, HmCmsJML079_04423, HmCmsJML146_01154, HmCmsJML204_02229, HmCmsJML236_03431, HW43_20810, MJ49_14450, MS6198_36560, MS8345_03519, NCTC10082_02969, NCTC10089_00628, NCTC10090_03661, NCTC10764_04007, NCTC10766_02930, NCTC10767_01659, NCTC10865_00812, NCTC10963_00588, NCTC11022_03335, NCTC11181_02870, NCTC12650_00863, NCTC13216_01221, NCTC13846_00637, NCTC7927_00693, NCTC7928_02343, NCTC8009_01696, NCTC8179_06096, NCTC8621_00648, NCTC8959_03135, NCTC8960_03211, NCTC9001_05629, NCTC9007_04393, NCTC9036_00679, NCTC9044_01214, NCTC9045_00716, NCTC9050_03744, NCTC9055_02513, NCTC9058_01245, NCTC9062_02553, NCTC9111_00997, NCTC9119_00654, NCTC9434_00565, NCTC9701_00708, NCTC9703_05099, NCTC9706_02867, NCTC9777_02133, NCTC9969_00790, PGD_03946, PU06_19955, RG28_20970, RK56_014715, RX35_04257, SAMEA3472043_01802, SAMEA3472044_03331, SAMEA3472047_01873, SAMEA3472055_00399, SAMEA3472056_03073, SAMEA3472070_01171, SAMEA3472080_00946, SAMEA3472090_00549, SAMEA3472108_00101, SAMEA3472110_01542, SAMEA3472112_01526, SAMEA3472114_00482, SAMEA3472147_01879, SAMEA3484427_00607, SAMEA3484429_00735, SAMEA3484434_03594, SAMEA3485101_01711, SAMEA3752372_01780, SAMEA3752553_01186, SAMEA3752557_01874, SAMEA3752559_03272, SAMEA3752620_02342, SAMEA3753064_02937, SAMEA3753097_03303, SAMEA3753164_02301, SAMEA3753290_00147, SAMEA3753300_02130, SK85_03483, SY51_18280, U12A_03406, U14A_03403, UC41_24430, UN86_15485, UN91_15510, WQ89_06745, WR15_24545, YDC107_1843 Production host: Escherichia coli (E. coli) / References: UniProt: A0A0J3VRH1 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Type: solution Contents: 600 uM [U-99% 13C; U-99% 15N] RimP, 10 mM HEPES, 6 mM MgCl2, 30 mM NH4Cl, 75 uM TCEP, 90% H2O/10% D2O Label: sample1 / Solvent system: 90% H2O/10% D2O | ||||||||||||||||||||||||
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Sample |
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Sample conditions | Ionic strength: 40 mM / Label: condition_1 / pH: 7.6 / Pressure: 1 atm / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement |
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NMR representative | Selection criteria: lowest energy | |||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 120 / Conformers submitted total number: 20 |