EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Dual Functions of a Rubisco Activase in Metabolic Repair and Recruitment to Carboxysomes.
ジャーナル・号・ページ	Cell, Vol. 183, Issue 2, Page 457-473.e20, Year 2020
掲載日	2020年10月15日
著者	Mirkko Flecken / Huping Wang / Leonhard Popilka / F Ulrich Hartl / Andreas Bracher / Manajit Hayer-Hartl /
PubMed 要旨	Rubisco, the key enzyme of CO fixation in photosynthesis, is prone to inactivation by inhibitory sugar phosphates. Inhibited Rubisco undergoes conformational repair by the hexameric AAA+ chaperone ...Rubisco, the key enzyme of CO fixation in photosynthesis, is prone to inactivation by inhibitory sugar phosphates. Inhibited Rubisco undergoes conformational repair by the hexameric AAA+ chaperone Rubisco activase (Rca) in a process that is not well understood. Here, we performed a structural and mechanistic analysis of cyanobacterial Rca, a close homolog of plant Rca. In the Rca:Rubisco complex, Rca is positioned over the Rubisco catalytic site under repair and pulls the N-terminal tail of the large Rubisco subunit (RbcL) into the hexamer pore. Simultaneous displacement of the C terminus of the adjacent RbcL opens the catalytic site for inhibitor release. An alternative interaction of Rca with Rubisco is mediated by C-terminal domains that resemble the small Rubisco subunit. These domains, together with the N-terminal AAA+ hexamer, ensure that Rca is packaged with Rubisco into carboxysomes. The cyanobacterial Rca is a dual-purpose protein with functions in Rubisco repair and carboxysome organization.
リンク	Cell / PubMed:32979320
手法	EM (単粒子) / X線回折
解像度	1.38 - 8.2 Å
構造データ	11028 6z1f EMDB-11028, PDB-6z1f: CryoEM structure of Rubisco Activase with its substrate Rubisco from Nostoc sp. (strain PCC7120) 手法: EM (単粒子) / 解像度: 2.86 Å 11029 6z1g EMDB-11029, PDB-6z1g: CryoEM structure of the interaction between Rubisco Activase small-subunit-like (SSUL) domain with Rubisco from Nostoc sp. (strain PCC7120) 手法: EM (単粒子) / 解像度: 8.2 Å EMDB-11575: CryoEM Local map of Rubisco Activase from the complex with its substrate Rubisco from Nostoc sp. (strain PCC7120) 手法: EM (単粒子) / 解像度: 3.29 Å PDB-6has: Crystal Structure of the small subunit-like domain of Rubisco activase from Nostoc sp. (strain PCC 7120) 手法: X-RAY DIFFRACTION / 解像度: 1.38 Å PDB-6z1d: Crystal structure of the AAA domain of Rubisco Activase from Nostoc sp. (strain PCC 7120), Gadolinium complex 手法: X-RAY DIFFRACTION / 解像度: 2.705 Å PDB-6z1e: Crystal structure of the AAA domain of Rubisco Activase from Nostoc sp. (strain PCC 7120) 手法: X-RAY DIFFRACTION / 解像度: 2.454 Å
化合物	ChemComp-NI: NICKEL (II) ION / ニッケル ChemComp-HOH: WATER / 水 ChemComp-GD: GADOLINIUM ATOM / ガドリニウム ChemComp-CL: Unknown entry / クロリド / 塩化物 ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸 ChemComp-AGS: PHOSPHOTHIOPHOSPHORIC ACID-ADENYLATE ESTER / ATP-γ-S / ATP-gamma-S, エネルギー貯蔵分子類似体YM ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-CAP: 2-CARBOXYARABINITOL-1,5-DIPHOSPHATE / 2-カルボキシ-D-アラビニト-ル1,5-ビスりん酸
由来	nostoc sp. pcc 7120 = fachb-418 (バクテリア) nostoc sp. (strain pcc 7120 / sag 25.82 / utex 2576) (バクテリア)
キーワード	CHAPERONE (シャペロン) / alpha-beta structure / Rubisco (リブロース1,5-ビスリン酸カルボキシラーゼ/オキシゲナーゼ) / AAA+ domain / AAA+ / beta barrel (Βバレル) / PHOTOSYNTHESIS (光合成)