EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	In situ structure of the Caulobacter crescentus flagellar motor and visualization of binding of a CheY-homolog.
ジャーナル・号・ページ	Mol Microbiol, Vol. 114, Issue 3, Page 443-453, Year 2020
掲載日	2020年5月25日
著者	Florian M Rossmann / Isabelle Hug / Matteo Sangermani / Urs Jenal / Morgan Beeby /
PubMed 要旨	Bacterial flagellar motility is controlled by the binding of CheY proteins to the cytoplasmic switch complex of the flagellar motor, resulting in changes in swimming speed or direction. Despite its ...Bacterial flagellar motility is controlled by the binding of CheY proteins to the cytoplasmic switch complex of the flagellar motor, resulting in changes in swimming speed or direction. Despite its importance for motor function, structural information about the interaction between effector proteins and the motor are scarce. To address this gap in knowledge, we used electron cryotomography and subtomogram averaging to visualize such interactions inside Caulobacter crescentus cells. In C. crescentus, several CheY homologs regulate motor function for different aspects of the bacterial lifestyle. We used subtomogram averaging to image binding of the CheY family protein CleD to the cytoplasmic Cring switch complex, the control center of the flagellar motor. This unambiguously confirmed the orientation of the motor switch protein FliM and the binding of a member of the CheY protein family to the outside rim of the C ring. We also uncovered previously unknown structural elaborations of the alphaproteobacterial flagellar motor, including two novel periplasmic ring structures, and the stator ring harboring eleven stator units, adding to our growing catalog of bacterial flagellar diversity.
リンク	Mol Microbiol / PubMed:32449846 / PubMed Central
手法	EM (サブトモグラム平均)
解像度	82.0 - 110.0 Å
構造データ	EMDB-10943: In situ structure of the Caulobacter crescentus flagellar motor and visualization of binding of a CheY-homolog 手法: EM (サブトモグラム平均) / 解像度: 82.0 Å EMDB-10945: In situ structure of the Caulobacter crescentus flagellar motor and visualization of binding of a CheY-homolog 手法: EM (サブトモグラム平均) / 解像度: 82.0 Å EMDB-10949: In situ structure of the Caulobacter crescentus flagellar motor and visualization of binding of a CheY-homolog 手法: EM (サブトモグラム平均) / 解像度: 82.0 Å EMDB-10950: In situ structure of the Caulobacter crescentus flagellar motor and visualization of binding of a CheY-homolog 手法: EM (サブトモグラム平均) / 解像度: 100.0 Å EMDB-10955: In situ structure of the Caulobacter crescentus flagellar motor and visualization of binding of a CheY-homolog 手法: EM (サブトモグラム平均) / 解像度: 110.0 Å EMDB-10956: In situ structure of the Caulobacter crescentus flagellar motor and visualization of binding of a CheY-homolog 手法: EM (サブトモグラム平均) / 解像度: 110.0 Å EMDB-10957: In situ structure of the Caulobacter crescentus flagellar motor and visualization of binding of a CheY-homolog 手法: EM (サブトモグラム平均) / 解像度: 110.0 Å
由来	Caulobacter vibrioides CB15 (バクテリア)