EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM structures of holo condensin reveal a subunit flip-flop mechanism.
ジャーナル・号・ページ	Nat Struct Mol Biol, Vol. 27, Issue 8, Page 743-751, Year 2020
掲載日	2020年7月13日
著者	Byung-Gil Lee / Fabian Merkel / Matteo Allegretti / Markus Hassler / Christopher Cawood / Léa Lecomte / Francis J O'Reilly / Ludwig R Sinn / Pilar Gutierrez-Escribano / Marc Kschonsak / Sol Bravo / Takanori Nakane / Juri Rappsilber / Luis Aragon / Martin Beck / Jan Löwe / Christian H Haering /
PubMed 要旨	Complexes containing a pair of structural maintenance of chromosomes (SMC) family proteins are fundamental for the three-dimensional (3D) organization of genomes in all domains of life. The ...Complexes containing a pair of structural maintenance of chromosomes (SMC) family proteins are fundamental for the three-dimensional (3D) organization of genomes in all domains of life. The eukaryotic SMC complexes cohesin and condensin are thought to fold interphase and mitotic chromosomes, respectively, into large loop domains, although the underlying molecular mechanisms have remained unknown. We used cryo-EM to investigate the nucleotide-driven reaction cycle of condensin from the budding yeast Saccharomyces cerevisiae. Our structures of the five-subunit condensin holo complex at different functional stages suggest that ATP binding induces the transition of the SMC coiled coils from a folded-rod conformation into a more open architecture. ATP binding simultaneously triggers the exchange of the two HEAT-repeat subunits bound to the SMC ATPase head domains. We propose that these steps result in the interconversion of DNA-binding sites in the catalytic core of condensin, forming the basis of the DNA translocation and loop-extrusion activities.
リンク	Nat Struct Mol Biol / PubMed:32661420 / PubMed Central
手法	EM (単粒子)
解像度	4.2 - 9.1 Å
構造データ	10944 6yvd EMDB-10944, PDB-6yvd: Head segment of the S.cerevisiae condensin holocomplex in presence of ATP 手法: EM (単粒子) / 解像度: 7.6 Å EMDB-10947: Condensin complex from S.cerevisiae ATP-free apo non-engaged state: focused refinement on arm segment 手法: EM (単粒子) / 解像度: 5.3 Å EMDB-10948: Condensin complex from S.cerevisiae ATP-free apo non-engaged state: focused refinement on head segment 手法: EM (単粒子) / 解像度: 4.2 Å 10951 6yvu EMDB-10951: Condensin complex from S.cerevisiae ATP-free apo non-engaged state: overall map PDB-6yvu: Condensin complex from S.cerevisiae ATP-free apo non-engaged state 手法: EM (単粒子) / 解像度: 7.5 Å 10952 6yvv EMDB-10952: Condensin complex from S.cerevisiae ATP-free apo bridged state: focused refinement on head segment PDB-6yvv: Condensin complex from S.cerevisiae ATP-free apo bridged state 手法: EM (単粒子) / 解像度: 7.5 Å EMDB-10953: Condensin complex from S.cerevisiae ATP-free apo bridged state: focused refinement on arm segment 手法: EM (単粒子) / 解像度: 7.8 Å EMDB-10954: Condensin complex from S.cerevisiae ATP-free apo bridged state: overall map 手法: EM (単粒子) / 解像度: 9.1 Å EMDB-10964: Rod-shaped arm segment of the S.cerevisiae condensin complex in presence of ATP 手法: EM (単粒子) / 解像度: 8.2 Å
由来	Saccharomyces cerevisiae S288C (パン酵母) saccharomyces cerevisiae (strain atcc 204508 / s288c) (パン酵母) Saccharomyces cerevisiae (パン酵母)
キーワード	CELL CYCLE / Condensin chromosome condensation SMC protein / essential for the functional organization of genomes