EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Type III ATP synthase is a symmetry-deviated dimer that induces membrane curvature through tetramerization.
ジャーナル・号・ページ	Nat Commun, Vol. 11, Issue 1, Page 5342, Year 2020
掲載日	2020年10月22日
著者	Rasmus Kock Flygaard / Alexander Mühleip / Victor Tobiasson / Alexey Amunts /
PubMed 要旨	Mitochondrial ATP synthases form functional homodimers to induce cristae curvature that is a universal property of mitochondria. To expand on the understanding of this fundamental phenomenon, we ...Mitochondrial ATP synthases form functional homodimers to induce cristae curvature that is a universal property of mitochondria. To expand on the understanding of this fundamental phenomenon, we characterized the unique type III mitochondrial ATP synthase in its dimeric and tetrameric form. The cryo-EM structure of a ciliate ATP synthase dimer reveals an unusual U-shaped assembly of 81 proteins, including a substoichiometrically bound ATPTT2, 40 lipids, and co-factors NAD and CoQ. A single copy of subunit ATPTT2 functions as a membrane anchor for the dimeric inhibitor IF. Type III specific linker proteins stably tie the ATP synthase monomers in parallel to each other. The intricate dimer architecture is scaffolded by an extended subunit-a that provides a template for both intra- and inter-dimer interactions. The latter results in the formation of tetramer assemblies, the membrane part of which we determined to 3.1 Å resolution. The structure of the type III ATP synthase tetramer and its associated lipids suggests that it is the intact unit propagating the membrane curvature.
リンク	Nat Commun / PubMed:33093501 / PubMed Central
手法	EM (単粒子)
解像度	2.5 - 3.1 Å
構造データ	10857 6ynv EMDB-10857, PDB-6ynv: Cryo-EM structure of Tetrahymena thermophila mitochondrial ATP synthase - Fo-wing region 手法: EM (単粒子) / 解像度: 2.8 Å 10858 6ynw EMDB-10858, PDB-6ynw: Cryo-EM structure of Tetrahymena thermophila mitochondrial ATP synthase - central stalk/cring 手法: EM (単粒子) / 解像度: 3.1 Å 10859 6ynx EMDB-10859, PDB-6ynx: Cryo-EM structure of Tetrahymena thermophila mitochondrial ATP synthase - Fo-subcomplex 手法: EM (単粒子) / 解像度: 2.5 Å 10860 6yny EMDB-10860: Cryo-EM structure of Tetrahymena thermophila mitochondrial ATP synthase - F1Fo dimer PDB-6yny: Cryo-EM structure of Tetrahymena thermophila mitochondrial ATP synthase - F1Fo composite dimer model 手法: EM (単粒子) / 解像度: 2.7 Å 10861 6ynz EMDB-10861: Cryo-EM structure of Tetrahymena thermophila mitochondrial ATP synthase - F1Fo tetramer PDB-6ynz: Cryo-EM structure of Tetrahymena thermophila mitochondrial ATP synthase - F1Fo composite tetramer model 手法: EM (単粒子) / 解像度: 3.1 Å 10862 6yo0 EMDB-10862, PDB-6yo0: Cryo-EM structure of Tetrahymena thermophila mitochondrial ATP synthase - F1/peripheral stalk 手法: EM (単粒子) / 解像度: 2.9 Å
化合物	ChemComp-NAD: NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NADYM / ニコチンアミドアデニンジヌクレオチド ChemComp-CDL: CARDIOLIPIN / リン脂質YM / Cardiolipin ChemComp-PC1: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / リン脂質YM / ホスファチジルコリン ChemComp-PO4: PHOSPHATE ION / ホスファ-ト / リン酸塩 ChemComp-UQ8: Ubiquinone-8 / ユビキノン8 ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM / アデノシン三リン酸 ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-PEE: 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine / DOPE, リン脂質YM / Discrete optimized protein energy ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸
由来	tetrahymena thermophila (真核生物)
キーワード	MEMBRANE PROTEIN (膜タンパク質) / mitochondria (ミトコンドリア) / ATP synthase (ATP合成酵素) / oxidoreductase (酸化還元酵素) / NAD / central stalk / c-ring (土星の環) / Fo-subcomplex / IF1 dimer / F1Fo dimer / F1Fo tetramer / F1-subcomplex / peripheral stalk / IF1