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-Structure paper
タイトル | Structure and Function of Stator Units of the Bacterial Flagellar Motor. |
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ジャーナル・号・ページ | Cell, Vol. 183, Issue 1, Page 244-257.e16, Year 2020 |
掲載日 | 2020年10月1日 |
著者 | Mònica Santiveri / Aritz Roa-Eguiara / Caroline Kühne / Navish Wadhwa / Haidai Hu / Howard C Berg / Marc Erhardt / Nicholas M I Taylor / |
PubMed 要旨 | Many bacteria use the flagellum for locomotion and chemotaxis. Its bidirectional rotation is driven by a membrane-embedded motor, which uses energy from the transmembrane ion gradient to generate ...Many bacteria use the flagellum for locomotion and chemotaxis. Its bidirectional rotation is driven by a membrane-embedded motor, which uses energy from the transmembrane ion gradient to generate torque at the interface between stator units and rotor. The structural organization of the stator unit (MotAB), its conformational changes upon ion transport, and how these changes power rotation of the flagellum remain unknown. Here, we present ~3 Å-resolution cryoelectron microscopy reconstructions of the stator unit in different functional states. We show that the stator unit consists of a dimer of MotB surrounded by a pentamer of MotA. Combining structural data with mutagenesis and functional studies, we identify key residues involved in torque generation and present a detailed mechanistic model for motor function and switching of rotational direction. |
リンク | Cell / PubMed:32931735 |
手法 | EM (単粒子) |
解像度 | 2.98 - 6.45 Å |
構造データ | EMDB-10828, PDB-6ykm: EMDB-10829, PDB-6ykp: EMDB-10830, PDB-6ykr: EMDB-10831: EMDB-10832: |
化合物 | ChemComp-HOH: |
由来 |
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キーワード | MEMBRANE PROTEIN / Bacterial flagellar motor / stator unit / locomotion / proton transport / ion transport |