EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Architecture and regulation of an enterobacterial cellulose secretion system.
ジャーナル・号・ページ	Sci Adv, Vol. 7, Issue 5, Year 2021
掲載日	2021年1月27日
著者	Wiem Abidi / Samira Zouhir / Meryem Caleechurn / Stéphane Roche / Petya Violinova Krasteva /
PubMed 要旨	Many free-living and pathogenic enterobacteria secrete biofilm-promoting cellulose using a multicomponent, envelope-embedded Bcs secretion system under the control of intracellular second messenger c- ...Many free-living and pathogenic enterobacteria secrete biofilm-promoting cellulose using a multicomponent, envelope-embedded Bcs secretion system under the control of intracellular second messenger c-di-GMP. The molecular understanding of system assembly and cellulose secretion has been largely limited to the crystallographic studies of a distantly homologous BcsAB synthase tandem and a low-resolution reconstruction of an assembled macrocomplex that encompasses most of the inner membrane and cytosolic subunits and features an atypical layered architecture. Here, we present cryo-EM structures of the assembled Bcs macrocomplex, as well as multiple crystallographic snapshots of regulatory Bcs subcomplexes. The structural and functional data uncover the mechanism of asymmetric secretion system assembly and periplasmic crown polymerization and reveal unexpected subunit stoichiometry, multisite c-di-GMP recognition, and ATP-dependent regulation.
リンク	Sci Adv / PubMed:33563593 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	1.59 - 5.9 Å
構造データ	10799 6yg8 EMDB-10799: Pentameric structure of the cellulose synthase regulator protein BcsB from Escherichia coli PDB-6yg8: Cryo-EM structure of a BcsB pentamer in the context of an assembled Bcs macrocomplex 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-11356: Structure of detergent-extracted full-length E.coli BcsB 手法: EM (単粒子) / 解像度: 5.9 Å EMDB-11836: Cryo-EM density map corresponding to BcsRQAB subcomplex 手法: EM (単粒子) / 解像度: 4.5 Å PDB-6yar: Crystal structure of a Selenium-derivatized complex of the bacterial cellulose secretion regulators BcsR and BcsQ, crystallized in the presence of AppCp 手法: X-RAY DIFFRACTION / 解像度: 1.9 Å PDB-6yay: Crystal structure of a Selenium-derivatized complex of the bacterial cellulose secretion regulators BcsR and BcsQ, crystallized in the presence of ADP 手法: X-RAY DIFFRACTION / 解像度: 2.09 Å PDB-6yb3: Crystal structure of a native BcsRQ complex purified and crystallized in the absence of nucleotide 手法: X-RAY DIFFRACTION / 解像度: 1.59 Å PDB-6yb5: Orthorhombic crystal structure of a native BcsRQ complex crystallized in the presence of ADP 手法: X-RAY DIFFRACTION / 解像度: 1.59 Å PDB-6ybb: Crystal structure of a native BcsE (217-523) - BcsR-BcsQ (R156E mutant) complex with c-di-GMP and ATP bound 手法: X-RAY DIFFRACTION / 解像度: 2.9 Å PDB-6ybu: Crystal structure of a native BcsE (349-523) RQ complex with c-di-GMP and ATP bound 手法: X-RAY DIFFRACTION / 解像度: 2.49 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM ChemComp-HOH: WATER ChemComp-C2E: 9,9'-[(2R,3R,3aS,5S,7aR,9R,10R,10aS,12S,14aR)-3,5,10,12-tetrahydroxy-5,12-dioxidooctahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-2,9-diyl]bis(2-amino-1,9-dihydro-6H-purin-6-one) / c-di-GMP ChemComp-GOL*: GLYCEROL / グリセロ-ル
由来	escherichia coli (大腸菌) escherichia coli (strain k12) (大腸菌)
キーワード	SIGNALING PROTEIN / Bacterial biofilms / Bacterial cellulose / Bacterial secretion system / ATP binding protein / c-di-GMP binding protein / regulator protein