EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Template-free detection and classification of membrane-bound complexes in cryo-electron tomograms.
ジャーナル・号・ページ	Nat Methods, Vol. 17, Issue 2, Page 209-216, Year 2020
掲載日	2020年1月6日
著者	Antonio Martinez-Sanchez / Zdravko Kochovski / Ulrike Laugks / Johannes Meyer Zum Alten Borgloh / Saikat Chakraborty / Stefan Pfeffer / Wolfgang Baumeister / Vladan Lučić /
PubMed 要旨	With faithful sample preservation and direct imaging of fully hydrated biological material, cryo-electron tomography provides an accurate representation of molecular architecture of cells. However, ...With faithful sample preservation and direct imaging of fully hydrated biological material, cryo-electron tomography provides an accurate representation of molecular architecture of cells. However, detection and precise localization of macromolecular complexes within cellular environments is aggravated by the presence of many molecular species and molecular crowding. We developed a template-free image processing procedure for accurate tracing of complex networks of densities in cryo-electron tomograms, a comprehensive and automated detection of heterogeneous membrane-bound complexes and an unsupervised classification (PySeg). Applications to intact cells and isolated endoplasmic reticulum (ER) allowed us to detect and classify small protein complexes. This classification provided sufficiently homogeneous particle sets and initial references to allow subsequent de novo subtomogram averaging. Spatial distribution analysis showed that ER complexes have different localization patterns forming nanodomains. Therefore, this procedure allows a comprehensive detection and structural analysis of complexes in situ.
リンク	Nat Methods / PubMed:31907446
手法	EM (サブトモグラム平均) / EM (トモグラフィー)
解像度	14.0 - 39.08 Å
構造データ	EMDB-0074: Template-free detection and classification of microsomal membrane bound complexes 手法: EM (サブトモグラム平均) / 解像度: 18.0 Å EMDB-0075: Template-free detection and classification of microsomal membrane bound complexes 手法: EM (サブトモグラム平均) / 解像度: 21.0 Å EMDB-0084: Template-free detection and classification of microsomal membrane bound complexes 手法: EM (サブトモグラム平均) / 解像度: 22.0 Å EMDB-0085: Template-free detection and classification of microsomal membrane bound complexes 手法: EM (サブトモグラム平均) / 解像度: 22.0 Å EMDB-0086: Template-free detection and classification of microsomal membrane bound complexes 手法: EM (サブトモグラム平均) / 解像度: 14.0 Å EMDB-0087: Template-free detection and classification of microsomal membrane bound complexes 手法: EM (サブトモグラム平均) / 解像度: 16.0 Å EMDB-10432: In situ ER membrane bound ribosome 手法: EM (サブトモグラム平均) / 解像度: 30.0 Å EMDB-10433: In situ ER membrane bound translocon-ribosome 手法: EM (サブトモグラム平均) / 解像度: 36.48 Å EMDB-10434: In situ ER membrane bound ribosome-free translocon 手法: EM (サブトモグラム平均) / 解像度: 39.08 Å EMDB-10435: Putative in situ ER membrane bound major histocompatibility peptide loading complex (PLC) 手法: EM (サブトモグラム平均) / 解像度: 34.2 Å EMDB-10436: Putative in situ ER membrane bound major histocompatibility peptide loading complex (PLC) 手法: EM (サブトモグラム平均) / 解像度: 32.18 Å EMDB-10437: Putative in situ ER membrane bound inositol trisphosphate receptor complex (IP3R) 手法: EM (サブトモグラム平均) / 解像度: 34.2 Å EMDB-10439: Example of tomogram used for in situ template-free membrane bound complexes determination 手法: EM (トモグラフィー) EMDB-10449: ER Microsome in Fig. 3 (Martinez et al., Nature Methods) 手法: EM (トモグラフィー) EMDB-10450: Endoplasmic Reticulum microsome used for Videos in (Martinez-Sanchez et al., Nature Methods) 手法: EM (トモグラフィー) EMDB-10451: ER microsome released with the code in (Martinez-Sanchez et al., Nature Methods) 手法: EM (トモグラフィー)
由来	Canis lupus familiaris (イヌ) Mus musculus (ハツカネズミ)