EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural insights into the activation of metabotropic glutamate receptors.
ジャーナル・号・ページ	Nature, Vol. 566, Issue 7742, Page 79-84, Year 2019
掲載日	2019年1月23日
著者	Antoine Koehl / Hongli Hu / Dan Feng / Bingfa Sun / Yan Zhang / Michael J Robertson / Matthew Chu / Tong Sun Kobilka / Toon Laeremans / Jan Steyaert / Jeffrey Tarrasch / Somnath Dutta / Rasmus Fonseca / William I Weis / Jesper M Mathiesen / Georgios Skiniotis / Brian K Kobilka /
PubMed 要旨	Metabotropic glutamate receptors are family C G-protein-coupled receptors. They form obligate dimers and possess extracellular ligand-binding Venus flytrap domains, which are linked by cysteine-rich ...Metabotropic glutamate receptors are family C G-protein-coupled receptors. They form obligate dimers and possess extracellular ligand-binding Venus flytrap domains, which are linked by cysteine-rich domains to their 7-transmembrane domains. Spectroscopic studies show that signalling is a dynamic process, in which large-scale conformational changes underlie the transmission of signals from the extracellular Venus flytraps to the G protein-coupling domains-the 7-transmembrane domains-in the membrane. Here, using a combination of X-ray crystallography, cryo-electron microscopy and signalling studies, we present a structural framework for the activation mechanism of metabotropic glutamate receptor subtype 5. Our results show that agonist binding at the Venus flytraps leads to a compaction of the intersubunit dimer interface, thereby bringing the cysteine-rich domains into close proximity. Interactions between the cysteine-rich domains and the second extracellular loops of the receptor enable the rigid-body repositioning of the 7-transmembrane domains, which come into contact with each other to initiate signalling.
リンク	Nature / PubMed:30675062 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	3.262 - 7.9 Å
構造データ	0345 6n51 EMDB-0345, PDB-6n51: Metabotropic Glutamate Receptor 5 bound to L-quisqualate and Nb43 手法: EM (単粒子) / 解像度: 4.0 Å 0346 6n52 EMDB-0346, PDB-6n52: Metabotropic Glutamate Receptor 5 Apo Form 手法: EM (単粒子) / 解像度: 4.0 Å EMDB-0347: Apo form metabotropic glutamate receptor 5 with Nanobody 43 手法: EM (単粒子) / 解像度: 7.9 Å PDB-6n4x: Metabotropic Glutamate Receptor 5 Apo Form Ligand Binding Domain 手法: X-RAY DIFFRACTION / 解像度: 4.0 Å PDB-6n4y: Metabotropic Glutamate Receptor 5 Extracellular Domain with Nb43 手法: X-RAY DIFFRACTION / 解像度: 3.262 Å PDB-6n50: Metabotropic Glutamate Receptor 5 Extracellular Domain in Complex with Nb43 and L-quisqualic acid 手法: X-RAY DIFFRACTION / 解像度: 3.751 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン / N-アセチルグルコサミン ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-QUS: (S)-2-AMINO-3-(3,5-DIOXO-[1,2,4]OXADIAZOLIDIN-2-YL)-PROPIONIC ACID / キスカル酸 / アゴニスト*YM / キスカル酸
由来	homo sapiens (ヒト) lama glama (ラマ)
キーワード	MEMBRANE PROTEIN (膜タンパク質) / Cell Surface Receptor (細胞表面受容体) / Cell Surface Receptor Nanobody / SIGNALING PROTEIN