EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural insights into influenza A virus ribonucleoproteins reveal a processive helical track as transcription mechanism.
ジャーナル・号・ページ	Nat Microbiol, Vol. 5, Issue 5, Page 727-734, Year 2020
掲載日	2020年3月9日
著者	Rocío Coloma / Rocío Arranz / José M de la Rosa-Trevín / Carlos O S Sorzano / Sandie Munier / Diego Carlero / Nadia Naffakh / Juan Ortín / Jaime Martín-Benito /
PubMed 要旨	The influenza virus genome consists of eight viral ribonucleoproteins (vRNPs), each consisting of a copy of the polymerase, one of the genomic RNA segments and multiple copies of the nucleoprotein ...The influenza virus genome consists of eight viral ribonucleoproteins (vRNPs), each consisting of a copy of the polymerase, one of the genomic RNA segments and multiple copies of the nucleoprotein arranged in a double helical conformation. vRNPs are macromolecular machines responsible for messenger RNA synthesis and genome replication, that is, the formation of progeny vRNPs. Here, we describe the structural basis of the transcription process. The mechanism, which we call the 'processive helical track', is based on the extreme flexibility of the helical part of the vRNP that permits a sliding movement between both antiparallel nucleoprotein-RNA strands, thereby allowing the polymerase to move over the genome while bound to both RNA ends. Accordingly, we demonstrate that blocking this movement leads to inhibition of vRNP transcriptional activity. This mechanism also reveals a critical role of the nucleoprotein in maintaining the double helical structure throughout the copying process to make the RNA template accessible to the polymerase.
リンク	Nat Microbiol / PubMed:32152587
手法	EM (らせん対称)
解像度	10.0 - 24.0 Å
構造データ	0175 6h9g EMDB-0175: Helical part of the influenza A virus ribonucleoprotein. Conformation 1. PDB-6h9g: Influenza A nucleoprotein docked into 3D helical structure of the wild type ribonucleoprotein complex obtained using cryoEM. Conformation 1. 手法: EM (らせん対称) / 解像度: 11.0 Å 4412 6i54 EMDB-4412: Helical part of the influenza A virus ribonucleoprotein. Conformation 2. PDB-6i54: Influenza A nucleoprotein docked into 3D helical structure of the wild type ribonucleoprotein complex obtained using cryoEM. Conformation 2. 手法: EM (らせん対称) / 解像度: 10.0 Å 4423 6i7b EMDB-4423: Helical part of the influenza A virus ribonucleoprotein. Conformation 3. PDB-6i7b: Influenza A nucleoprotein docked into 3D helical structure of the wild type ribonucleoprotein complex obtained using cryoEM. Conformation 3. 手法: EM (らせん対称) / 解像度: 10.0 Å 4426 6i7m EMDB-4426: Helical part of the influenza A virus ribonucleoprotein. Conformation 4. PDB-6i7m: Influenza A nucleoprotein docked into 3D helical structure of the wild type ribonucleoprotein complex obtained using cryoEM. Conformation 4. 手法: EM (らせん対称) / 解像度: 10.0 Å 4430 6i85 EMDB-4430: Helical part of the influenza A virus ribonucleoprotein. Conformation 5. PDB-6i85: Influenza A nucleoprotein docked into the 3D helical structure of the wild type ribonucleoprotein complex obtained using cryoEM. Conformation 5. 手法: EM (らせん対称) / 解像度: 24.0 Å
由来	influenza a virus (A型インフルエンザウイルス) influenza a virus (strain a/wilson-smith/1933 h1n1) (A型インフルエンザウイルス) influenza a virus (a/wilson-smith/1933(h1n1)) (A型インフルエンザウイルス)
キーワード	VIRAL PROTEIN / Influenza A virus Ribonucleoprotein RNA binding protein / Influenza A virus / Ribonucleoprotein / RNA binding protein / RNA binding protein.