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-Structure paper
タイトル | The Mla system and glycerophospholipid transport to the outer membrane. |
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ジャーナル・号・ページ | Elife, Vol. 8, Year 2019 |
掲載日 | 2019年1月14日 |
著者 | Cassandra Kamischke / Junping Fan / Julien Bergeron / Hemantha D Kulasekara / Zachary D Dalebroux / Anika Burrell / Justin M Kollman / Samuel I Miller / |
PubMed 要旨 | The outer membrane (OM) of Gram-negative bacteria serves as a selective permeability barrier that allows entry of essential nutrients while excluding toxic compounds, including antibiotics. The OM is ...The outer membrane (OM) of Gram-negative bacteria serves as a selective permeability barrier that allows entry of essential nutrients while excluding toxic compounds, including antibiotics. The OM is asymmetric and contains an outer leaflet of lipopolysaccharides (LPS) or lipooligosaccharides (LOS) and an inner leaflet of glycerophospholipids (GPL). We screened transposon mutants and identified a number of mutants with OM defects, including an ABC transporter system homologous to the Mla system in We further show that this opportunistic, antibiotic-resistant pathogen uses this multicomponent protein complex and ATP hydrolysis at the inner membrane to promote GPL export to the OM. The broad conservation of the Mla system in Gram-negative bacteria suggests the system may play a conserved role in OM biogenesis. The importance of the Mla system to OM integrity and antibiotic sensitivity suggests that its components may serve as new antimicrobial therapeutic targets. |
リンク | Elife / PubMed:30638443 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 8.7 Å |
構造データ | |
由来 |
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キーワード | PROTEIN TRANSPORT / Membrane protein Antibiotic resistance ABC transporter |