EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	FusC, a member of the M16 protease family acquired by bacteria for iron piracy against plants.
ジャーナル・号・ページ	PLoS Biol, Vol. 16, Issue 8, Page e2006026, Year 2018
掲載日	2018年8月2日
著者	Rhys Grinter / Iain D Hay / Jiangning Song / Jiawei Wang / Don Teng / Vijay Dhanesakaran / Jonathan J Wilksch / Mark R Davies / Dene Littler / Simone A Beckham / Ian R Henderson / Richard A Strugnell / Gordon Dougan / Trevor Lithgow /
PubMed 要旨	Iron is essential for life. Accessing iron from the environment can be a limiting factor that determines success in a given environmental niche. For bacteria, access of chelated iron from the ...Iron is essential for life. Accessing iron from the environment can be a limiting factor that determines success in a given environmental niche. For bacteria, access of chelated iron from the environment is often mediated by TonB-dependent transporters (TBDTs), which are β-barrel proteins that form sophisticated channels in the outer membrane. Reports of iron-bearing proteins being used as a source of iron indicate specific protein import reactions across the bacterial outer membrane. The molecular mechanism by which a folded protein can be imported in this way had remained mysterious, as did the evolutionary process that could lead to such a protein import pathway. How does the bacterium evolve the specificity factors that would be required to select and import a protein encoded on another organism's genome? We describe here a model whereby the plant iron-bearing protein ferredoxin can be imported across the outer membrane of the plant pathogen Pectobacterium by means of a Brownian ratchet mechanism, thereby liberating iron into the bacterium to enable its growth in plant tissues. This import pathway is facilitated by FusC, a member of the same protein family as the mitochondrial processing peptidase (MPP). The Brownian ratchet depends on binding sites discovered in crystal structures of FusC that engage a linear segment of the plant protein ferredoxin. Sequence relationships suggest that the bacterial gene encoding FusC has previously unappreciated homologues in plants and that the protein import mechanism employed by the bacterium is an evolutionary echo of the protein import pathway in plant mitochondria and plastids.
リンク	PLoS Biol / PubMed:30071011 / PubMed Central
手法	SAS (X-ray synchrotron) / X線回折
解像度	1.9 - 2.7 Å
構造データ	SASDD27: The ferredoxin protease, FusC, E83A mutant + 50 µM Arabidopsis ferredoxin 手法: SAXS/SANS SASDD37: The ferredoxin protease, FusC, E83A mutant + 70 µM Arabidopsis ferredoxin 手法: SAXS/SANS SASDD47: The ferredoxin protease, FusC, E83A mutant + 100 µM Arabidopsis ferredoxin 手法: SAXS/SANS SASDD57: The ferredoxin protease, FusC, E83A mutant + 150 µM Arabidopsis ferredoxin 手法: SAXS/SANS SASDD67: The ferredoxin protease, FusC, E83A mutant + 200 µM Arabidopsis ferredoxin 手法: SAXS/SANS SASDD77: The ferredoxin protease, FusC, E83A mutant + 300 µM Arabidopsis ferredoxin 手法: SAXS/SANS SASDDQ6: The ferredoxin protease, FusC (Ferredoxin Protease, FusC) 手法: SAXS/SANS SASDDR6: The ferredoxin protease, FusC, E83A mutant (Ferredoxin protease E83A mutant, FusC E83A) 手法: SAXS/SANS SASDDS6: The ferredoxin protease, FusC, with Arabidopsis ferredoxin (co-SEC-SAXS) 手法: SAXS/SANS SASDDT6: The ferredoxin protease, FusC, E83A mutant with Arabidopsis ferredoxin (co-SEC-SAXS) 手法: SAXS/SANS SASDDU6: The ferredoxin protease, FusC, E83A mutant (Ferredoxin protease E83A mutant, FusC E83A) 手法: SAXS/SANS SASDDV6: The ferredoxin protease, FusC, E83A mutant + 3 µM Arabidopsis ferredoxin 手法: SAXS/SANS SASDDW6: The ferredoxin protease, FusC, E83A mutant + 5 µM Arabidopsis ferredoxin 手法: SAXS/SANS SASDDX6: The ferredoxin protease, FusC, E83A mutant + 10 µM Arabidopsis ferredoxin 手法: SAXS/SANS SASDDY6: The ferredoxin protease, FusC, E83A mutant + 20 µM Arabidopsis ferredoxin 手法: SAXS/SANS SASDDZ6: The ferredoxin protease, FusC, E83A mutant + 30 µM Arabidopsis ferredoxin 手法: SAXS/SANS PDB-6b03: The crystal structure of the ferredoxin protease FusC in complex with its substrate plant ferredoxin 手法: X-RAY DIFFRACTION / 解像度: 2.7 Å PDB-6b05: The Crystal Structure of the Ferredoxin Protease FusC E83A mutant in complex with Arabidopsis Ferredoxin 手法: X-RAY DIFFRACTION / 解像度: 1.9 Å PDB-6brs: The Crystal Structure of the Ferredoxin Protease FusC in complex with Arabidopsis Ferredoxin, Ethylmercury phosphate soaked dataset 手法: X-RAY DIFFRACTION / 解像度: 2.3 Å
化合物	ChemComp-HOH: WATER ChemComp-ZN: Unknown entry ChemComp-PO4: PHOSPHATE ION / ホスファ-ト ChemComp-HG: Unknown entry
由来	pectobacterium atrosepticum scri1043 (バクテリア) arabidopsis thaliana (シロイヌナズナ) pectobacterium atrosepticum (strain scri 1043 / atcc baa-672) (バクテリア)
キーワード	HYDROLASE / M16 Protease / Ferredoxin cleavage / Iron Acquisition / Ferredoxin Binding