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- SASDDR6: The ferredoxin protease, FusC, E83A mutant (Ferredoxin protease E... -
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Open data
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Basic information
Entry | ![]() |
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![]() | The ferredoxin protease, FusC, E83A mutant
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Function / homology | ![]() |
Biological species | ![]() |
![]() | ![]() Title: FusC, a member of the M16 protease family acquired by bacteria for iron piracy against plants. Authors: Rhys Grinter / Iain D Hay / Jiangning Song / Jiawei Wang / Don Teng / Vijay Dhanesakaran / Jonathan J Wilksch / Mark R Davies / Dene Littler / Simone A Beckham / Ian R Henderson / Richard A ...Authors: Rhys Grinter / Iain D Hay / Jiangning Song / Jiawei Wang / Don Teng / Vijay Dhanesakaran / Jonathan J Wilksch / Mark R Davies / Dene Littler / Simone A Beckham / Ian R Henderson / Richard A Strugnell / Gordon Dougan / Trevor Lithgow / ![]() ![]() Abstract: Iron is essential for life. Accessing iron from the environment can be a limiting factor that determines success in a given environmental niche. For bacteria, access of chelated iron from the ...Iron is essential for life. Accessing iron from the environment can be a limiting factor that determines success in a given environmental niche. For bacteria, access of chelated iron from the environment is often mediated by TonB-dependent transporters (TBDTs), which are β-barrel proteins that form sophisticated channels in the outer membrane. Reports of iron-bearing proteins being used as a source of iron indicate specific protein import reactions across the bacterial outer membrane. The molecular mechanism by which a folded protein can be imported in this way had remained mysterious, as did the evolutionary process that could lead to such a protein import pathway. How does the bacterium evolve the specificity factors that would be required to select and import a protein encoded on another organism's genome? We describe here a model whereby the plant iron-bearing protein ferredoxin can be imported across the outer membrane of the plant pathogen Pectobacterium by means of a Brownian ratchet mechanism, thereby liberating iron into the bacterium to enable its growth in plant tissues. This import pathway is facilitated by FusC, a member of the same protein family as the mitochondrial processing peptidase (MPP). The Brownian ratchet depends on binding sites discovered in crystal structures of FusC that engage a linear segment of the plant protein ferredoxin. Sequence relationships suggest that the bacterial gene encoding FusC has previously unappreciated homologues in plants and that the protein import mechanism employed by the bacterium is an evolutionary echo of the protein import pathway in plant mitochondria and plastids. |
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Structure visualization
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Downloads & links
-Data source
SASBDB page | ![]() |
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-Related structure data
Related structure data | ![]() 6b03C ![]() 6b05C ![]() 6brsC C: citing same article ( |
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Similar structure data | Similarity search - Function & homology ![]() |
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External links
Related items in Molecule of the Month |
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-Models
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Sample
![]() | Name: The ferredoxin protease, FusC, E83A mutant / Specimen concentration: 10 mg/ml |
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Buffer | Name: 20 mM Tris HCl, 150 nM NaCl, 0.02 % NaAzide, 5% Glycerol pH: 7.8 |
Entity #1084 | Name: FusC E83A / Type: protein / Description: Ferredoxin protease E83A mutant / Formula weight: 101.195 / Num. of mol.: 1 / Source: Pectobacterium atrosepticum SCRI1043 / References: UniProt: Q6D8U3 Sequence: EEIKSPLPVF KEGTLANGFR YTLVQLEGPK TRVDIRLIVD VGSIDEKDNE SGVAHMVAHM VFRASDAFPQ GVSTELHKQG WGRGQSYNAV TNYERTMYMM SPPKGNLDLG ATLQALSQMT GHAKLLQSDL DDERKIILEE WRGKLGVAER MNQQRVQAIR HDSRYPSRPV ...Sequence: EEIKSPLPVF KEGTLANGFR YTLVQLEGPK TRVDIRLIVD VGSIDEKDNE SGVAHMVAHM VFRASDAFPQ GVSTELHKQG WGRGQSYNAV TNYERTMYMM SPPKGNLDLG ATLQALSQMT GHAKLLQSDL DDERKIILEE WRGKLGVAER MNQQRVQAIR HDSRYPSRPV IGTEESINDT PASVLQDFYQ RWYHPSNMRL MIIGDITPAD AEREIQRYFA ALPNVAVPTR DYYEPLLKPQ LKVARLQDSQ SGSSQVSFVY RFNDKDAFGQ SEYRHRLLTQ ITMSAVTRQV RRQKAELPQD ASSLVVRKSD IGKTTAALGF FANVMPGGHD AAISAVLKEI ERFKRYPLNE QDITEITSDI REVAQRMSVT PETREFADWV QQLTIVWQQD RPYVGSQQRG KDALEALDTI KGEDVNRHWQ RWLASPDTLA QFSVPGATPF TLPKPDAISK LQKQWALATL APLRLEEKKI IPELPSVTQS GKRTAVKTFA AQKVEQWQLS NGDRVVWLRA PEAGKKVYLT ATSQAGFMAT AMNPWQAQLA SQLVNQSGPA TWSGESLSNW KKEKTLSLSI DQEADQLTLS GTAPTEQLAS LFGLYRELNV APGIDPDVMK ESMMSLARQK ANDDQSVGGK RASEMTKLRF GEPAWQQPEI AELKKISAPA LLSQWHKAAS APVTYYLIAD MPATQLLPQV ERYLATIPRQ PASEVKQHLA LSGKREATSA INVEPRADIL TWSFTPHAWT PQAAVQVSIA RNIASKYLKT SLRDDALGIY RMRVDSELED KKQRIETEVS FTSAPERAQE LWTLAEQAFS ELPTKITQQD VDEQKAQFIR AEKGRQGDLT TIQRRLILSY RHYNDPRYLS NASKLADSIT LESVRAMSAK LYNPDNRVLY ITLPQEVKE |
-Experimental information
Beam | Instrument name: Australian Synchrotron SAXS/WAXS / City: Melbourne / 国: Australia ![]() | ||||||||||||||||||||||||||||||
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Detector | Name: Pilatus 1M / Pixsize x: 172 mm | ||||||||||||||||||||||||||||||
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Distance distribution function P(R) |
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Result | Comments: Additional SEC parameters: Column type: GE Healthcare Superdex S200 10/300; Flow rate: 0.4 ml/min; Sample injection concentration: 10 mg/ml; Injection volume: 100 µl.
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