EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM structures of CTP synthase filaments reveal mechanism of pH-sensitive assembly during budding yeast starvation.
ジャーナル・号・ページ	Elife, Vol. 10, Year 2021
掲載日	2021年11月4日
著者	Jesse M Hansen / Avital Horowitz / Eric M Lynch / Daniel P Farrell / Joel Quispe / Frank DiMaio / Justin M Kollman /
PubMed 要旨	Many metabolic enzymes self-assemble into micron-scale filaments to organize and regulate metabolism. The appearance of these assemblies often coincides with large metabolic changes as in ...Many metabolic enzymes self-assemble into micron-scale filaments to organize and regulate metabolism. The appearance of these assemblies often coincides with large metabolic changes as in development, cancer, and stress. Yeast undergo cytoplasmic acidification upon starvation, triggering the assembly of many metabolic enzymes into filaments. However, it is unclear how these filaments assemble at the molecular level and what their role is in the yeast starvation response. CTP Synthase (CTPS) assembles into metabolic filaments across many species. Here, we characterize in vitro polymerization and investigate in vivo consequences of CTPS assembly in yeast. Cryo-EM structures reveal a pH-sensitive assembly mechanism and highly ordered filament bundles that stabilize an inactive state of the enzyme, features unique to yeast CTPS. Disruption of filaments in cells with non-assembly or pH-insensitive mutations decreases growth rate, reflecting the importance of regulated CTPS filament assembly in homeotstasis.
リンク	Elife / PubMed:34734801 / PubMed Central
手法	EM (単粒子)
解像度	2.8 - 7.3 Å
構造データ	24497 7rkh EMDB-24497, PDB-7rkh: Yeast CTP Synthase (URA8) tetramer bound to ATP/UTP at neutral pH 手法: EM (単粒子) / 解像度: 2.8 Å 24512 7rl0 EMDB-24512, PDB-7rl0: Yeast CTP Synthase (URA8) Filament bound to ATP/UTP at low pH 手法: EM (単粒子) / 解像度: 2.8 Å 24516 7rl5 EMDB-24516, PDB-7rl5: Yeast CTP Synthase (URA8) filament bound to CTP at low pH 手法: EM (単粒子) / 解像度: 3.8 Å 24560 7rmc EMDB-24560, PDB-7rmc: Yeast CTP Synthase (Ura7) filament bound to CTP at low pH 手法: EM (単粒子) / 解像度: 3.5 Å 24566 7rmf EMDB-24566, PDB-7rmf: Substrate-bound Ura7 filament at low pH 手法: EM (単粒子) / 解像度: 7.3 Å 24575 7rmk EMDB-24575, PDB-7rmk: Yeast CTP Synthase (Ura7) Bundle bound to substrates at low pH 手法: EM (単粒子) / 解像度: 6.6 Å 24576 7rmo EMDB-24576, PDB-7rmo: Yeast CTP Synthase (Ura7) Bundle bound to Products at low pH 手法: EM (単粒子) / 解像度: 7.0 Å 24578 7rmv EMDB-24578, PDB-7rmv: Yeast CTP Synthase (Ura7) H360R Filament bound to Substrates 手法: EM (単粒子) / 解像度: 6.7 Å 24579 7rnl EMDB-24579, PDB-7rnl: Yeast CTP Synthase (Ura7) H360R Filament bound to Substrates 手法: EM (単粒子) / 解像度: 3.7 Å 24581 7rnr EMDB-24581, PDB-7rnr: Yeast CTP Synthase (Ura8) Bundle Bound to Substrates at Low pH 手法: EM (単粒子) / 解像度: 3.3 Å
化合物	ChemComp-UTP: URIDINE 5'-TRIPHOSPHATE / UTP / UTPYM / ウリジン三リン酸 ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM / アデノシン三リン酸 ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-CTP: CYTIDINE-5'-TRIPHOSPHATE / CTP / シチジン三リン酸
由来	saccharomyces cerevisiae (パン酵母)
キーワード	PROTEIN FIBRIL / glutaminase (グルタミナーゼ) / amido-ligase / nucleotide metabolism (ヌクレオチド) / amino-ligase / glutaminase and amino-ligase / glutaminase and amido-ligase