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-Structure paper
タイトル | A Workflow for Protein Structure Determination From Thin Crystal Lamella by Micro-Electron Diffraction. |
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ジャーナル・号・ページ | Front Mol Biosci, Vol. 7, Page 179, Year 2020 |
掲載日 | 2020年8月4日 |
著者 | Emma V Beale / David G Waterman / Corey Hecksel / Jason van Rooyen / James B Gilchrist / James M Parkhurst / Felix de Haas / Bart Buijsse / Gwyndaf Evans / Peijun Zhang / |
PubMed 要旨 | MicroED has recently emerged as a powerful method for the analysis of biological structures at atomic resolution. This technique has been largely limited to protein nanocrystals which grow either as ...MicroED has recently emerged as a powerful method for the analysis of biological structures at atomic resolution. This technique has been largely limited to protein nanocrystals which grow either as needles or plates measuring only a few hundred nanometers in thickness. Furthermore, traditional microED data processing uses established X-ray crystallography software that is not optimized for handling compound effects that are unique to electron diffraction data. Here, we present an integrated workflow for microED, from sample preparation by cryo-focused ion beam milling, through data collection with a standard Ceta-D detector, to data processing using the DIALS software suite, thus enabling routine atomic structure determination of protein crystals of any size and shape using microED. We demonstrate the effectiveness of the workflow by determining the structure of proteinase K to 2.0 Å resolution and show the advantage of using protein crystal lamellae over nanocrystals. |
リンク | Front Mol Biosci / PubMed:32850967 / PubMed Central |
手法 | EM (電子線結晶学) |
解像度 | 2.004 - 2.701 Å |
構造データ | PDB-6zet: PDB-6zeu: PDB-6zev: |
化合物 | ChemComp-CA: ChemComp-HOH: |
由来 |
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キーワード | HYDROLASE (加水分解酵素) / Protease (プロテアーゼ) / Serine protease (セリンプロテアーゼ) |