EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Ciliate mitoribosome illuminates evolutionary steps of mitochondrial translation.
ジャーナル・号・ページ	Elife, Vol. 9, Year 2020
掲載日	2020年6月18日
著者	Victor Tobiasson / Alexey Amunts /
PubMed 要旨	To understand the steps involved in the evolution of translation, we used , a ciliate with high coding capacity of the mitochondrial genome, as the model organism and characterized its mitochondrial ...To understand the steps involved in the evolution of translation, we used , a ciliate with high coding capacity of the mitochondrial genome, as the model organism and characterized its mitochondrial ribosome (mitoribosome) using cryo-EM. The structure of the mitoribosome reveals an assembly of 94-ribosomal proteins and four-rRNAs with an additional protein mass of ~700 kDa on the small subunit, while the large subunit lacks 5S rRNA. The structure also shows that the small subunit head is constrained, tRNA binding sites are formed by mitochondria-specific protein elements, conserved protein bS1 is excluded, and bacterial RNA polymerase binding site is blocked. We provide evidence for anintrinsic protein targeting system through visualization of mitochondria-specific mL105 by the exit tunnel that would facilitate the recruitment of a nascent polypeptide. Functional protein uS3m is encoded by three complementary genes from the nucleus and mitochondrion, establishing a link between genetic drift and mitochondrial translation. Finally, we reannotated nine open reading frames in the mitochondrial genome that code for mitoribosomal proteins.
リンク	Elife / PubMed:32553108 / PubMed Central
手法	EM (単粒子)
解像度	3.3 - 3.7 Å
構造データ	11032 6z1p EMDB-11032: The mitochondrial ribosome from Tetrahymena thermophila PDB-6z1p: Structure of the mitochondrial ribosome from Tetrahymena thermophila 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-11033: The mitochondrial ribosome from Tetrahymena thermophila, large subunit mask 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-11034: The mitochondrial ribosome from Tetrahymena thermophila, small subunit mask 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-11035: The mitochondrial ribosome from Tetrahymena thermophila, central protuberance mask 手法: EM (単粒子) / 解像度: 3.6 Å EMDB-11036: The mitochondrial ribosome from Tetrahymena thermophila, L7/L12 stalk mask 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-11037: The mitochondrial ribosome from Tetrahymena thermophila, small subunit head mask 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-11038: The mitochondrial ribosome from Tetrahymena thermophila, small subunit back protuberance mask 手法: EM (単粒子) / 解像度: 3.6 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ZN: Unknown entry ChemComp-FES: FE2/S2 (INORGANIC) CLUSTER / 鉄・硫黄クラスター ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子*YM / アデノシン三リン酸
由来	Tetrahymena thermophila SB210 (真核生物) tetrahymena thermophila (strain sb210) (真核生物)
キーワード	RIBOSOME (リボソーム) / Mitochondrial ribosome / mitochondria (ミトコンドリア) / ciliate (繊毛虫) / tetrahymena (テトラヒメナ)