EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Molecular insight into RNA polymerase I promoter recognition and promoter melting.
ジャーナル・号・ページ	Nat Commun, Vol. 10, Issue 1, Page 5543, Year 2019
掲載日	2019年12月5日
著者	Yashar Sadian / Florence Baudin / Lucas Tafur / Brice Murciano / Rene Wetzel / Felix Weis / Christoph W Müller /
PubMed 要旨	RNA polymerase I (Pol I) assembles with core factor (CF) and Rrn3 on the rDNA core promoter for transcription initiation. Here, we report cryo-EM structures of closed, intermediate and open Pol I ...RNA polymerase I (Pol I) assembles with core factor (CF) and Rrn3 on the rDNA core promoter for transcription initiation. Here, we report cryo-EM structures of closed, intermediate and open Pol I initiation complexes from 2.7 to 3.7 Å resolution to visualize Pol I promoter melting and to structurally and biochemically characterize the recognition mechanism of Pol I promoter DNA. In the closed complex, double-stranded DNA runs outside the DNA-binding cleft. Rotation of CF and upstream DNA with respect to Pol I and Rrn3 results in the spontaneous loading and opening of the promoter followed by cleft closure and positioning of the Pol I A49 tandem winged helix domain (tWH) onto DNA. Conformational rearrangement of A49 tWH leads to a clash with Rrn3 to initiate complex disassembly and promoter escape. Comprehensive insight into the Pol I transcription initiation cycle allows comparisons with promoter opening by Pol II and Pol III.
リンク	Nat Commun / PubMed:31804486 / PubMed Central
手法	EM (単粒子)
解像度	2.7 - 4.0 Å
構造データ	10006 6rui EMDB-10006, PDB-6rui: RNA Polymerase I Pre-initiation complex DNA opening intermediate 2 手法: EM (単粒子) / 解像度: 2.7 Å 10007 6ruo EMDB-10007, PDB-6ruo: RNA Polymerase I Open Complex conformation 1 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-10019: RNA Polymerase I Open Complex conformation 2 focused refinement on CF 手法: EM (単粒子) / 解像度: 3.0 Å EMDB-10020: RNA Polymerase I Open Complex conformation 2 focused refinement on Pol 手法: EM (単粒子) / 解像度: 2.96 Å 10038 6rwe EMDB-10038, PDB-6rwe: RNA Polymerase I Open Complex conformation 2 手法: EM (単粒子) / 解像度: 3.0 Å 4982 6rqh EMDB-4982, PDB-6rqh: RNA Polymerase I Closed Conformation 1 (CC1) 手法: EM (単粒子) / 解像度: 3.7 Å 4984 6rql EMDB-4984, PDB-6rql: RNA Polymerase I Closed Conformation 2 (CC2) 手法: EM (単粒子) / 解像度: 2.9 Å 4985 6rqt EMDB-4985, PDB-6rqt: RNA Polymerase I-tWH-Rrn3-DNA 手法: EM (単粒子) / 解像度: 4.0 Å 4987 6rrd EMDB-4987, PDB-6rrd: RNA Polymerase I Pre-initiation complex DNA opening intermediate 1 手法: EM (単粒子) / 解像度: 3.1 Å
化合物	ChemComp-ZN: Unknown entry
由来	saccharomyces cerevisiae (パン酵母) synthetic construct (人工物) saccharomyces cerevisiae (strain atcc 204508 / s288c) (パン酵母)
キーワード	TRANSCRIPTION (転写 (生物学)) / RNA Polymerase I (RNAポリメラーゼI) / Pre-initiation complex / PIC / Closed Complex / CC / Core Factor / CF / Rrn3 / POL1 / tandem winged helix / tWH / Promoter escape / Open Complex / OC / DNA opening