EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural assembly of the tailed bacteriophage ϕ29.
ジャーナル・号・ページ	Nat Commun, Vol. 10, Issue 1, Page 2366, Year 2019
掲載日	2019年5月30日
著者	Jingwei Xu / Dianhong Wang / Miao Gui / Ye Xiang /
PubMed 要旨	The mature virion of the tailed bacteriophage ϕ29 is an ~33 MDa complex that contains more than 450 subunits of seven structural proteins assembling into a prolate head and a short non-contractile ...The mature virion of the tailed bacteriophage ϕ29 is an ~33 MDa complex that contains more than 450 subunits of seven structural proteins assembling into a prolate head and a short non-contractile tail. Here, we report the near-atomic structures of the ϕ29 pre-genome packaging head (prohead), the mature virion and the genome-emptied virion. Structural comparisons suggest local rotation or oscillation of the head-tail connector upon DNA packaging and release. Termination of the DNA packaging occurs through pressure-dependent correlative positional and conformational changes in the connector. The funnel-shaped tail lower collar attaches the expanded narrow end of the connector and has a 180-Å long, 24-strand β barrel narrow stem tube that undergoes conformational changes upon genome release. The appendages form an interlocked assembly attaching the tail around the collar. The membrane active long loops at the distal end of the tail knob exit during the late stage of infection and form the cone-shaped tip of a largely hydrophobic helix barrel, prepared for membrane penetration.
リンク	Nat Commun / PubMed:31147544 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	1.8 - 5.0 Å
構造データ	4655 6qvk EMDB-4655, PDB-6qvk: The cryo-EM structure of bacteriophage phi29 prohead 手法: EM (単粒子) / 解像度: 3.6 Å 4662 6qx7 EMDB-4662, PDB-6qx7: The cryo-EM structure of connector in bacteriophage phi29 prohead 手法: EM (単粒子) / 解像度: 3.8 Å 4677 6qyd EMDB-4677, PDB-6qyd: Cryo-EM structure of the head in mature bacteriophage phi29 手法: EM (単粒子) / 解像度: 3.2 Å 4678 6qyj EMDB-4678, PDB-6qyj: The cryo-EM structure of the connector of the mature bacteriophage phi29 手法: EM (単粒子) / 解像度: 3.4 Å 4679 6qym EMDB-4679, PDB-6qym: The cryo-EM structure of the connector of the genome empited bacteriophage phi29 手法: EM (単粒子) / 解像度: 3.6 Å 4680 6qyz EMDB-4680, PDB-6qyz: The cryo-EM structure of prohead RNA in bacteriophage phi29 prohead 手法: EM (単粒子) / 解像度: 4.6 Å 4681 6qz0 EMDB-4681, PDB-6qz0: The cryo-EM structure of the head of the genome empited bacteriophage phi29 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-4682: The cryo-EM structure of the tail knob in the bacteriophage phi29 手法: EM (単粒子) / 解像度: 5.0 Å EMDB-4683: The cryo-EM structure of the tail knob in genome empited bacteriophage phi29 手法: EM (単粒子) / 解像度: 4.2 Å 4684 6qz9 EMDB-4684, PDB-6qz9: The cryo-EM structure of the collar complex and tail axis in bacteriophage phi29 手法: EM (単粒子) / 解像度: 3.3 Å 4685 6qzf EMDB-4685, PDB-6qzf: The cryo-EM structure of the collar complex and tail axis in genome emptied bacteriophage phi29 手法: EM (単粒子) / 解像度: 3.8 Å PDB-6qyy: The crystal structure of head fiber gp8.5 N base in bacteriophage phi29 手法: X-RAY DIFFRACTION / 解像度: 1.8 Å
化合物	ChemComp-SO4: SULFATE ION / 硫酸ジアニオン / 硫酸塩 ChemComp-HOH: WATER / 水
由来	bacillus phage phi29 (ファージ)
キーワード	VIRUS (ウイルス) / bacteriophage (ファージ) / phi29 / prohead / cryo-EM (低温電子顕微鏡法) / mature virus / genome emptied virion / mature virion