EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural Basis of RNA Polymerase I Transcription Initiation.
ジャーナル・号・ページ	Cell, Vol. 169, Issue 1, Page 120-131.e22, Year 2017
掲載日	2017年3月23日
著者	Christoph Engel / Tobias Gubbey / Simon Neyer / Sarah Sainsbury / Christiane Oberthuer / Carlo Baejen / Carrie Bernecky / Patrick Cramer /
PubMed 要旨	Transcription initiation at the ribosomal RNA promoter requires RNA polymerase (Pol) I and the initiation factors Rrn3 and core factor (CF). Here, we combine X-ray crystallography and cryo-electron ...Transcription initiation at the ribosomal RNA promoter requires RNA polymerase (Pol) I and the initiation factors Rrn3 and core factor (CF). Here, we combine X-ray crystallography and cryo-electron microscopy (cryo-EM) to obtain a molecular model for basal Pol I initiation. The three-subunit CF binds upstream promoter DNA, docks to the Pol I-Rrn3 complex, and loads DNA into the expanded active center cleft of the polymerase. DNA unwinding between the Pol I protrusion and clamp domains enables cleft contraction, resulting in an active Pol I conformation and RNA synthesis. Comparison with the Pol II system suggests that promoter specificity relies on a distinct "bendability" and "meltability" of the promoter sequence that enables contacts between initiation factors, DNA, and polymerase.
リンク	Cell / PubMed:28340337
手法	EM (単粒子) / X線回折
解像度	3.2 - 7.7 Å
構造データ	3590 5n5y EMDB-3590, PDB-5n5y: Cryo-EM structure of RNA polymerase I in complex with Rrn3 and Core Factor (Orientation III) 手法: EM (単粒子) / 解像度: 7.7 Å 3591 5n5z EMDB-3591, PDB-5n5z: Cryo-EM structure of RNA polymerase I in complex with Rrn3 and Core Factor (Orientation II) 手法: EM (単粒子) / 解像度: 7.7 Å 3592 5n60 EMDB-3592, PDB-5n60: Cryo-EM structure of RNA polymerase I in complex with Rrn3 and Core Factor (Orientation I) 手法: EM (単粒子) / 解像度: 7.7 Å 3593 5n61 EMDB-3593, PDB-5n61: RNA polymerase I initially transcribing complex 手法: EM (単粒子) / 解像度: 3.4 Å 3594 5n61 EMDB-3594: RNA polymerase I initially transcribing complex with visible tandem Winged Helix domain PDB-5n61: RNA polymerase I initially transcribing complex 手法: EM (単粒子) / 解像度: 6.9 Å PDB-5o7x: CRYSTAL STRUCTURE OF S. CEREVISIAE CORE FACTOR AT 3.2A RESOLUTION 手法: X-RAY DIFFRACTION / 解像度: 3.2 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-SO4: SULFATE ION / 硫酸ジアニオン / 硫酸塩 ChemComp-MG: Unknown entry / マグネシウムジカチオン
由来	saccharomyces cerevisiae (パン酵母) Baker's yeast (パン酵母) saccharomyces cerevisiae (strain atcc 204508 / s288c) (パン酵母)
キーワード	TRANSCRIPTION (転写 (生物学)) / RNA polymerase I initiation / TRANSFERASE (転移酵素) / RNA polymerase I (RNAポリメラーゼI) / initially transcribing complex / CORE FACTOR