EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structure of a yeast spliceosome at 3.6-angstrom resolution.
ジャーナル・号・ページ	Science, Vol. 349, Issue 6253, Page 1182-1191, Year 2015
掲載日	2015年9月11日
著者	Chuangye Yan / Jing Hang / Ruixue Wan / Min Huang / Catherine C L Wong / Yigong Shi /
PubMed 要旨	Splicing of precursor messenger RNA (pre-mRNA) in yeast is executed by the spliceosome, which consists of five small nuclear ribonucleoproteins (snRNPs), NTC (nineteen complex), NTC-related proteins ...Splicing of precursor messenger RNA (pre-mRNA) in yeast is executed by the spliceosome, which consists of five small nuclear ribonucleoproteins (snRNPs), NTC (nineteen complex), NTC-related proteins (NTR), and a number of associated enzymes and cofactors. Here, we report the three-dimensional structure of a Schizosaccharomyces pombe spliceosome at 3.6-angstrom resolution, revealed by means of single-particle cryogenic electron microscopy. This spliceosome contains U2 and U5 snRNPs, NTC, NTR, U6 small nuclear RNA, and an RNA intron lariat. The atomic model includes 10,574 amino acids from 37 proteins and four RNA molecules, with a combined molecular mass of approximately 1.3 megadaltons. Spp42 (Prp8 in Saccharomyces cerevisiae), the key protein component of the U5 snRNP, forms a central scaffold and anchors the catalytic center. Both the morphology and the placement of protein components appear to have evolved to facilitate the dynamic process of pre-mRNA splicing. Our near-atomic-resolution structure of a central spliceosome provides a molecular framework for mechanistic understanding of pre-mRNA splicing.
リンク	Science / PubMed:26292707
手法	EM (単粒子)
解像度	3.3 - 6.89 Å
構造データ	6413 3jb9 EMDB-6413, PDB-3jb9: Cryo-EM structure of the yeast spliceosome at 3.6 angstrom resolution 手法: EM (単粒子) / 解像度: 3.6 Å 6414 3jb9 EMDB-6414: Local map for Spp42 region of the yeast spliceosome at 3.42 angstrom resolution PDB-3jb9: Cryo-EM structure of the yeast spliceosome at 3.6 angstrom resolution 手法: EM (単粒子) / 解像度: 3.42 Å 6415 3jb9 EMDB-6415: Local map for reduced Spp42 region of the yeast spliceosome at 3.52 angstrom resolution PDB-3jb9: Cryo-EM structure of the yeast spliceosome at 3.6 angstrom resolution 手法: EM (単粒子) / 解像度: 3.52 Å 6416 3jb9 EMDB-6416: Local map for Cwf10/Sm region of the yeast spliceosome at 3.30 angstrom resolution PDB-3jb9: Cryo-EM structure of the yeast spliceosome at 3.6 angstrom resolution 手法: EM (単粒子) / 解像度: 3.3 Å 6417 3jb9 EMDB-6417: Local map for reduced Cwf10/Sm region of the yeast spliceosome at 3.44 angstrom resolution PDB-3jb9: Cryo-EM structure of the yeast spliceosome at 3.6 angstrom resolution 手法: EM (単粒子) / 解像度: 3.44 Å 6418 3jb9 EMDB-6418: Local map for cetral region of the yeast spliceosome at 3.57 angstrom resolution PDB-3jb9: Cryo-EM structure of the yeast spliceosome at 3.6 angstrom resolution 手法: EM (単粒子) / 解像度: 3.57 Å 6419 3jb9 EMDB-6419: Local map for reduced central region of the yeast spliceosome at 3.96 angstrom resolution PDB-3jb9: Cryo-EM structure of the yeast spliceosome at 3.6 angstrom resolution 手法: EM (単粒子) / 解像度: 3.96 Å 6420 3jb9 EMDB-6420: Local map for Cwf17/Cwf8 region of the yeast spliceosome at 4.28 angstrom resolution PDB-3jb9: Cryo-EM structure of the yeast spliceosome at 3.6 angstrom resolution 手法: EM (単粒子) / 解像度: 4.28 Å 6421 3jb9 EMDB-6421: Local map for Cwf11/U2 region of the yeast spliceosome at 6.89 angstrom resolution PDB-3jb9: Cryo-EM structure of the yeast spliceosome at 3.6 angstrom resolution 手法: EM (単粒子) / 解像度: 6.89 Å
化合物	ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP / GDP, エネルギー貯蔵分子YM / グアノシン二リン酸 ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ZN: Unknown entry ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸
由来	schizosaccharomyces pombe (分裂酵母) schizosaccharomyces pombe 972h- (分裂酵母)
キーワード	RNA BINDING PROTEIN/RNA / Spliceosome (スプライセオソーム) / U2/U5/U6 / Lariat / RNA BINDING PROTEIN-RNA complex