EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Allosteric communication in the dynein motor domain.
ジャーナル・号・ページ	Cell, Vol. 159, Issue 4, Page 857-868, Year 2014
掲載日	2014年11月6日
著者	Gira Bhabha / Hui-Chun Cheng / Nan Zhang / Arne Moeller / Maofu Liao / Jeffrey A Speir / Yifan Cheng / Ronald D Vale /
PubMed 要旨	Dyneins power microtubule motility using ring-shaped, AAA-containing motor domains. Here, we report X-ray and electron microscopy (EM) structures of yeast dynein bound to different ATP analogs, which ...Dyneins power microtubule motility using ring-shaped, AAA-containing motor domains. Here, we report X-ray and electron microscopy (EM) structures of yeast dynein bound to different ATP analogs, which collectively provide insight into the roles of dynein's two major ATPase sites, AAA1 and AAA3, in the conformational change mechanism. ATP binding to AAA1 triggers a cascade of conformational changes that propagate to all six AAA domains and cause a large movement of the "linker," dynein's mechanical element. In contrast to the role of AAA1 in driving motility, nucleotide transitions in AAA3 gate the transmission of conformational changes between AAA1 and the linker, suggesting that AAA3 acts as a regulatory switch. Further structural and mutational studies also uncover a role for the linker in regulating the catalytic cycle of AAA1. Together, these results reveal how dynein's two major ATP-binding sites initiate and modulate conformational changes in the motor domain during motility.
リンク	Cell / PubMed:25417161 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	3.541 - 27.0 Å
構造データ	EMDB-6047: Cryo-EM structure of the dynein motor domain in the presence of ADP-AlF3 手法: EM (単粒子) / 解像度: 10.5 Å EMDB-6048: Cryo-EM structure of the dynein motor domain in the presence of ADP-AlF3 手法: EM (単粒子) / 解像度: 15.0 Å EMDB-6049: Cryo-EM structure of the yeast dynein motor domain in the presence of ADP-vanadate 手法: EM (単粒子) / 解像度: 9.0 Å EMDB-6050: Cryo-EM structure of the yeast dynein motor domain in the presence of ADP-vanadate 手法: EM (単粒子) / 解像度: 15.0 Å EMDB-6051: Cryo-EM structure of the yeast dynein motor domain in the presence of ADP-vanadate 手法: EM (単粒子) / 解像度: 14.0 Å EMDB-6052: Negative stain EM structure of the yeast dynein motor domain with stalk and MTBD in the presence of ADP-vanadate 手法: EM (単粒子) / 解像度: 24.0 Å EMDB-6053: Negative stain EM structure of the yeast dynein motor domain with stalk and MTBD in the presence of ADP-vanadate 手法: EM (単粒子) / 解像度: 27.0 Å EMDB-6054: Negative stain EM structure of the yeast dynein motor domain in the presence of ATP 手法: EM (単粒子) / 解像度: 17.0 Å EMDB-6055: Negative stain EM structure of the yeast dynein motor domain in the presence of ATP 手法: EM (単粒子) / 解像度: 18.0 Å EMDB-6056: Negative stain EM structure of the yeast dynein motor domain in the presence of ATP 手法: EM (単粒子) / 解像度: 15.0 Å EMDB-6058: Negative stain EM structure of the yeast dynein motor domain in the presence of ATP 手法: EM (単粒子) / 解像度: 19.0 Å EMDB-6059: Negative stain EM structure of the yeast dynein motor domain in the presence of ATP 手法: EM (単粒子) / 解像度: 16.0 Å EMDB-6060: Negative stain EM structure of the apo yeast dynein motor domain 手法: EM (単粒子) / 解像度: 16.0 Å EMDB-6061: Negative stain EM structure of the apo yeast dynein motor domain 手法: EM (単粒子) / 解像度: 25.0 Å EMDB-6062: Negative stain EM structure of the yeast dynein motor domain in the presence of AMPPNP 手法: EM (単粒子) / 解像度: 15.0 Å EMDB-6063: Negative stain EM structure of the yeast dynein motor domain in the presence of AMPPNP 手法: EM (単粒子) / 解像度: 15.0 Å EMDB-6064: Negative stain EM structure of the yeast dynein motor domain in the presence of AMPPNP 手法: EM (単粒子) / 解像度: 15.0 Å EMDB-6065: Negative stain EM structure of the yeast dynein motor domain with stalk and MTBD in the presence of ATP 手法: EM (単粒子) / 解像度: 23.0 Å EMDB-6066: Negative stain EM structure of the yeast dynein motor domain with stalk and MTBD in the presence of ATP 手法: EM (単粒子) / 解像度: 21.0 Å EMDB-6067: Negative stain EM structure of the yeast dynein motor domain with stalk and MTBD in the presence of ATP 手法: EM (単粒子) / 解像度: 23.0 Å EMDB-6068: Negative stain EM structure of the yeast dynein motor domain with stalk and MTBD in the presence of ATP 手法: EM (単粒子) / 解像度: 23.0 Å EMDB-6069: Negative stain EM structure of E2488Q mutant of the yeast dynein motor domain in the presence of ATP 手法: EM (単粒子) / 解像度: 20.0 Å EMDB-6070: Negative stain EM structure of E1849Q/E2488Q mutant of the yeast dynein motor domain in the presence of ATP 手法: EM (単粒子) / 解像度: 22.0 Å EMDB-6071: Negative stain EM structure of E1849Q/R2209A mutant of the yeast dynein motor domain in the presence of ATP 手法: EM (単粒子) / 解像度: 18.0 Å EMDB-6072: Negative stain EM structure of the yeast dynein motor domain with stalk and MTBD in the presence of ADP-AlF3 手法: EM (単粒子) / 解像度: 23.0 Å EMDB-6073: Negative stain EM structure of the yeast dynein motor domain with stalk and MTBD in the presence of ADP-AlF3 手法: EM (単粒子) / 解像度: 24.0 Å EMDB-6074: Negative stain EM structure of the yeast dynein motor domain in the presence of ADP 手法: EM (単粒子) / 解像度: 15.0 Å PDB-4w8f: Crystal structure of the dynein motor domain in the AMPPNP-bound state 手法: X-RAY DIFFRACTION / 解像度: 3.541 Å
化合物	ChemComp-ANP: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP / AMP-PNP, エネルギー貯蔵分子類似体YM ChemComp-MG*: Unknown entry / マグネシウムジカチオン
由来	saccharomyces cerevisiae (パン酵母) enterobacteria phage t4 (ファージ)
キーワード	MOTOR PROTEIN (モータータンパク質) / cytoplasmic dynein (ダイニン) / microtubule (微小管) / ATPase (ATPアーゼ) / AAA+ / AMPPNP