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Structure paper

タイトル	The cellular environment shapes the nuclear pore complex architecture.
ジャーナル・号・ページ	Nature, Vol. 598, Issue 7882, Page 667-671, Year 2021
掲載日	2021年10月13日
著者	Anthony P Schuller / Matthias Wojtynek / David Mankus / Meltem Tatli / Rafael Kronenberg-Tenga / Saroj G Regmi / Phat V Dip / Abigail K R Lytton-Jean / Edward J Brignole / Mary Dasso / Karsten Weis / Ohad Medalia / Thomas U Schwartz /
PubMed 要旨	Nuclear pore complexes (NPCs) create large conduits for cargo transport between the nucleus and cytoplasm across the nuclear envelope (NE). These multi-megadalton structures are composed of about ...Nuclear pore complexes (NPCs) create large conduits for cargo transport between the nucleus and cytoplasm across the nuclear envelope (NE). These multi-megadalton structures are composed of about thirty different nucleoporins that are distributed in three main substructures (the inner, cytoplasmic and nucleoplasmic rings) around the central transport channel. Here we use cryo-electron tomography on DLD-1 cells that were prepared using cryo-focused-ion-beam milling to generate a structural model for the human NPC in its native environment. We show that-compared with previous human NPC models obtained from purified NEs-the inner ring in our model is substantially wider; the volume of the central channel is increased by 75% and the nucleoplasmic and cytoplasmic rings are reorganized. Moreover, the NPC membrane exhibits asymmetry around the inner-ring complex. Using targeted degradation of Nup96, a scaffold nucleoporin of the cytoplasmic and nucleoplasmic rings, we observe the interdependence of each ring in modulating the central channel and maintaining membrane asymmetry. Our findings highlight the inherent flexibility of the NPC and suggest that the cellular environment has a considerable influence on NPC dimensions and architecture.
リンク	Nature / PubMed:34646014 / PubMed Central
手法	EM (サブトモグラム平均)
解像度	33.0 - 39.0 Å
構造データ	EMDB-12811: Segment of the cytoplasmic ring of the human nuclear pore complex 手法: EM (サブトモグラム平均) / 解像度: 33.0 Å EMDB-12812: Segment of the inner ring of the human nuclear pore complex 手法: EM (サブトモグラム平均) / 解像度: 38.0 Å EMDB-12813: Segment of the nucleoplasmic ring of the human nuclear pore complex 手法: EM (サブトモグラム平均) / 解像度: 39.0 Å 12814 7peq 7per EMDB-12814: In-cell human nuclear pore complex PDB-7peq: Model of the outer rings of the human nuclear pore complex PDB-7per: Model of the inner ring of the human nuclear pore complex 手法: EM (サブトモグラム平均) / 解像度: 35.0 Å
由来	homo sapiens (ヒト)
キーワード	TRANSPORT PROTEIN (運搬体タンパク質) / Nuclear Pore Complex (核膜孔) / NPC