+検索条件
-Structure paper
タイトル | Immunization expands B cells specific to HIV-1 V3 glycan in mice and macaques. |
---|---|
ジャーナル・号・ページ | Nature, Vol. 570, Issue 7762, Page 468-473, Year 2019 |
掲載日 | 2019年5月29日 |
著者 | Amelia Escolano / Harry B Gristick / Morgan E Abernathy / Julia Merkenschlager / Rajeev Gautam / Thiago Y Oliveira / Joy Pai / Anthony P West / Christopher O Barnes / Alexander A Cohen / Haoqing Wang / Jovana Golijanin / Daniel Yost / Jennifer R Keeffe / Zijun Wang / Peng Zhao / Kai-Hui Yao / Jens Bauer / Lilian Nogueira / Han Gao / Alisa V Voll / David C Montefiori / Michael S Seaman / Anna Gazumyan / Murillo Silva / Andrew T McGuire / Leonidas Stamatatos / Darrell J Irvine / Lance Wells / Malcolm A Martin / Pamela J Bjorkman / Michel C Nussenzweig / |
PubMed 要旨 | Broadly neutralizing monoclonal antibodies protect against infection with HIV-1 in animal models, suggesting that a vaccine that elicits these antibodies would be protective in humans. However, it ...Broadly neutralizing monoclonal antibodies protect against infection with HIV-1 in animal models, suggesting that a vaccine that elicits these antibodies would be protective in humans. However, it has not yet been possible to induce adequate serological responses by vaccination. Here, to activate B cells that express precursors of broadly neutralizing antibodies within polyclonal repertoires, we developed an immunogen, RC1, that facilitates the recognition of the variable loop 3 (V3)-glycan patch on the envelope protein of HIV-1. RC1 conceals non-conserved immunodominant regions by the addition of glycans and/or multimerization on virus-like particles. Immunization of mice, rabbits and rhesus macaques with RC1 elicited serological responses that targeted the V3-glycan patch. Antibody cloning and cryo-electron microscopy structures of antibody-envelope complexes confirmed that immunization with RC1 expands clones of B cells that carry the anti-V3-glycan patch antibodies, which resemble precursors of human broadly neutralizing antibodies. Thus, RC1 may be a suitable priming immunogen for sequential vaccination strategies in the context of polyclonal repertoires. |
リンク | Nature / PubMed:31142836 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.9 - 4.4 Å |
構造データ | EMDB-20175, PDB-6orn: EMDB-20176, PDB-6oro: EMDB-20177, PDB-6orp: EMDB-20178, PDB-6orq: |
化合物 | ChemComp-NAG: |
由来 |
|
キーワード | VIRAL PROTEIN/Immune System (ウイルス性) / HIV-1 broadly-neutralizing antibody / Env trimer structure / V3-glycan patch / cryo-EM (低温電子顕微鏡法) / RC1 / immunogen design / ANTIVIRAL PROTEIN-IMMUNE SYSTEM complex (抗ウイルス薬) / ANTIVIRAL PROTEIN / VIRAL PROTEIN-Immune System complex (ウイルス性) |