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Structure paper

タイトル	Visualization of translation termination intermediates trapped by the Apidaecin 137 peptide during RF3-mediated recycling of RF1.
ジャーナル・号・ページ	Nat Commun, Vol. 9, Issue 1, Page 3053, Year 2018
掲載日	2018年8月3日
著者	Michael Graf / Paul Huter / Cristina Maracci / Miroslav Peterek / Marina V Rodnina / Daniel N Wilson /
PubMed 要旨	During translation termination in bacteria, the release factors RF1 and RF2 are recycled from the ribosome by RF3. While high-resolution structures of the individual termination factors on the ...During translation termination in bacteria, the release factors RF1 and RF2 are recycled from the ribosome by RF3. While high-resolution structures of the individual termination factors on the ribosome exist, direct structural insight into how RF3 mediates dissociation of the decoding RFs has been lacking. Here we have used the Apidaecin 137 peptide to trap RF1 together with RF3 on the ribosome and visualize an ensemble of termination intermediates using cryo-electron microscopy. Binding of RF3 to the ribosome induces small subunit (SSU) rotation and swivelling of the head, yielding intermediate states with shifted P-site tRNAs and RF1 conformations. RF3 does not directly eject RF1 from the ribosome, but rather induces full rotation of the SSU that indirectly dislodges RF1 from its binding site. SSU rotation is coupled to the accommodation of the GTPase domain of RF3 on the large subunit (LSU), thereby promoting GTP hydrolysis and dissociation of RF3 from the ribosome.
リンク	Nat Commun / PubMed:30076302 / PubMed Central
手法	EM (単粒子)
解像度	3.8 - 4.4 Å
構造データ	0076 6gwt EMDB-0076, PDB-6gwt: Cryo-EM structure of an E. coli 70S ribosome in complex with RF3-GDPCP, RF1(GAQ) and Pint-tRNA (State I) 手法: EM (単粒子) / 解像度: 3.8 Å 0080 6gxm EMDB-0080, PDB-6gxm: Cryo-EM structure of an E. coli 70S ribosome in complex with RF3-GDPCP, RF1(GAQ) and Pint-tRNA (State II) 手法: EM (単粒子) / 解像度: 3.8 Å 0081 6gxn EMDB-0081, PDB-6gxn: Cryo-EM structure of an E. coli 70S ribosome in complex with RF3-GDPCP, RF1(GAQ) and Pint-tRNA (State III) 手法: EM (単粒子) / 解像度: 3.9 Å 0082 6gxo EMDB-0082, PDB-6gxo: Cryo-EM structure of a rotated E. coli 70S ribosome in complex with RF3-GDPCP, RF1(GAQ) and P/E-tRNA (State IV) 手法: EM (単粒子) / 解像度: 3.9 Å 0083 6gxp EMDB-0083, PDB-6gxp: Cryo-EM structure of a rotated E. coli 70S ribosome in complex with RF3-GDPCP(RF3-only) 手法: EM (単粒子) / 解像度: 4.4 Å
化合物	ChemComp-GCP: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / グアニリルメチレンジホスホン酸 / GMP-PCP, エネルギー貯蔵分子類似体*YM
由来	escherichia coli (大腸菌) apis mellifera (セイヨウミツバチ)
キーワード	RIBOSOME / single particle cryo-EM / GTPase / release factor RF1 / RF2 / RF3 / subunit rotation / translation termination