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Structure paper

タイトル	Structural basis for the initiation of eukaryotic transcription-coupled DNA repair.
ジャーナル・号・ページ	Nature, Vol. 551, Issue 7682, Page 653-657, Year 2017
掲載日	2017年11月30日
著者	Jun Xu / Indrajit Lahiri / Wei Wang / Adam Wier / Michael A Cianfrocco / Jenny Chong / Alissa A Hare / Peter B Dervan / Frank DiMaio / Andres E Leschziner / Dong Wang /
PubMed 要旨	Eukaryotic transcription-coupled repair (TCR) is an important and well-conserved sub-pathway of nucleotide excision repair that preferentially removes DNA lesions from the template strand that block ...Eukaryotic transcription-coupled repair (TCR) is an important and well-conserved sub-pathway of nucleotide excision repair that preferentially removes DNA lesions from the template strand that block translocation of RNA polymerase II (Pol II). Cockayne syndrome group B (CSB, also known as ERCC6) protein in humans (or its yeast orthologues, Rad26 in Saccharomyces cerevisiae and Rhp26 in Schizosaccharomyces pombe) is among the first proteins to be recruited to the lesion-arrested Pol II during the initiation of eukaryotic TCR. Mutations in CSB are associated with the autosomal-recessive neurological disorder Cockayne syndrome, which is characterized by progeriod features, growth failure and photosensitivity. The molecular mechanism of eukaryotic TCR initiation remains unclear, with several long-standing unanswered questions. How cells distinguish DNA lesion-arrested Pol II from other forms of arrested Pol II, the role of CSB in TCR initiation, and how CSB interacts with the arrested Pol II complex are all unknown. The lack of structures of CSB or the Pol II-CSB complex has hindered our ability to address these questions. Here we report the structure of the S. cerevisiae Pol II-Rad26 complex solved by cryo-electron microscopy. The structure reveals that Rad26 binds to the DNA upstream of Pol II, where it markedly alters its path. Our structural and functional data suggest that the conserved Swi2/Snf2-family core ATPase domain promotes the forward movement of Pol II, and elucidate key roles for Rad26 in both TCR and transcription elongation.
リンク	Nature / PubMed:29168508 / PubMed Central
手法	EM (単粒子)
解像度	4.5 - 10.0 Å
構造データ	EMDB-7038: RNA Pol II elongation complex bound to Rad26 手法: EM (単粒子) / 解像度: 5.8 Å 8735 5vvr EMDB-8735, PDB-5vvr: Ternary complex of RNA Pol II, transcription scaffold and Rad26 手法: EM (単粒子) / 解像度: 5.8 Å EMDB-8736: Structural Basis forEukaryotic Transcription-Coupled Repair Initiation 手法: EM (単粒子) / 解像度: 4.5 Å 8737 5vvs EMDB-8737, PDB-5vvs: RNA pol II elongation complex 手法: EM (単粒子) / 解像度: 6.4 Å EMDB-8885: RNA Pol II complex arrested by a CPD lesion 手法: EM (単粒子) / 解像度: 10.0 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-MG: Unknown entry / マグネシウムジカチオン
由来	saccharomyces cerevisiae (strain atcc 204508 / s288c) (パン酵母) saccharomyces cerevisiae (パン酵母)
キーワード	TRANSCRIPTION/RNA/DNA / complex / RNA polymerase (RNAポリメラーゼ) / CSB / transcription (転写 (生物学)) / TRANSCRIPTION-RNA-DNA complex