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Structure paper

タイトル	Structure of phycobilisome from the red alga Griffithsia pacifica.
ジャーナル・号・ページ	Nature, Vol. 551, Issue 7678, Page 57-63, Year 2017
掲載日	2017年11月2日
著者	Jun Zhang / Jianfei Ma / Desheng Liu / Song Qin / Shan Sun / Jindong Zhao / Sen-Fang Sui /
PubMed 要旨	Life on Earth depends on photosynthesis for its conversion of solar energy to chemical energy. Photosynthetic organisms have developed a variety of light-harvesting systems to capture sunlight. The ...Life on Earth depends on photosynthesis for its conversion of solar energy to chemical energy. Photosynthetic organisms have developed a variety of light-harvesting systems to capture sunlight. The largest light-harvesting complex is the phycobilisome (PBS), the main light-harvesting antenna in cyanobacteria and red algae. It is composed of phycobiliproteins and linker proteins but the assembly mechanisms and energy transfer pathways of the PBS are not well understood. Here we report the structure of a 16.8-megadalton PBS from a red alga at 3.5 Å resolution obtained by single-particle cryo-electron microscopy. We modelled 862 protein subunits, including 4 linkers in the core, 16 rod-core linkers and 52 rod linkers, and located a total of 2,048 chromophores. This structure reveals the mechanisms underlying specific interactions between linkers and phycobiliproteins, and the formation of linker skeletons. These results provide a firm structural basis for our understanding of complex assembly and the mechanisms of energy transfer within the PBS.
リンク	Nature / PubMed:29045394
手法	EM (単粒子)
解像度	3.43 - 4.29 Å
構造データ	EMDB-6758: Local map for the core region of the phycobilisome from the red alga Griffithsia pacifica 手法: EM (単粒子) / 解像度: 3.43 Å EMDB-6759: Local map for the Rd region of the phycobilisome from the red alga Griffithsia pacifica 手法: EM (単粒子) / 解像度: 3.68 Å EMDB-6760: Local map for the Ra region of the phycobilisome from the red alga Griffithsia pacifica 手法: EM (単粒子) / 解像度: 3.72 Å EMDB-6761: Local map for the Re region of the phycobilisome from the red alga Griffithsia pacifica 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-6762: Local map for the Rc region of the phycobilisome from the red alga Griffithsia pacifica 手法: EM (単粒子) / 解像度: 3.58 Å EMDB-6763: Local map for the Rb region of the phycobilisome from the red alga Griffithsia pacifica 手法: EM (単粒子) / 解像度: 3.59 Å EMDB-6764: Local map for the Rf region of the phycobilisome from the red alga Griffithsia pacifica 手法: EM (単粒子) / 解像度: 4.29 Å EMDB-6765: Local map for the Rg region of the phycobilisome from the red alga Griffithsia pacifica 手法: EM (単粒子) / 解像度: 3.74 Å EMDB-6766: Local map for the Ha region of the phycobilisome from the red alga Griffithsia pacifica 手法: EM (単粒子) / 解像度: 3.95 Å EMDB-6767: Local map for the Hb region of the phycobilisome from the red alga Griffithsia pacifica 手法: EM (単粒子) / 解像度: 3.87 Å EMDB-6768: Local map for the Hd region of the phycobilisome from the red alga Griffithsia pacifica 手法: EM (単粒子) / 解像度: 3.81 Å 6769 5y6p EMDB-6769: Overall map of the phycobilisome from the red alga Griffithsia pacifica PDB-5y6p: Structure of the phycobilisome from the red alga Griffithsia pacifica 手法: EM (単粒子) / 解像度: 3.5 Å
化合物	ChemComp-PEB: PHYCOERYTHROBILIN / Phycoerythrobilin ChemComp-CYC: PHYCOCYANOBILIN / フィコシアノビリン ChemComp-PUB: PHYCOUROBILIN / Phycourobilin ChemComp-PVN: PHYCOVIOLOBILIN
由来	griffithsia pacifica (真核生物)
キーワード	PHOTOSYNTHESIS (光合成) / phycobilisome (フィコビリソーム)