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Structure paper

タイトル	Mediator structure and rearrangements required for holoenzyme formation.
ジャーナル・号・ページ	Nature, Vol. 544, Issue 7649, Page 196-201, Year 2017
掲載日	2017年4月13日
著者	Kuang-Lei Tsai / Xiaodi Yu / Sneha Gopalan / Ti-Chun Chao / Ying Zhang / Laurence Florens / Michael P Washburn / Kenji Murakami / Ronald C Conaway / Joan W Conaway / Francisco J Asturias /
PubMed 要旨	The conserved Mediator co-activator complex has an essential role in the regulation of RNA polymerase II transcription in all eukaryotes. Understanding the structure and interactions of Mediator is ...The conserved Mediator co-activator complex has an essential role in the regulation of RNA polymerase II transcription in all eukaryotes. Understanding the structure and interactions of Mediator is crucial for determining how the complex influences transcription initiation and conveys regulatory information to the basal transcription machinery. Here we present a 4.4 Å resolution cryo-electron microscopy map of Schizosaccharomyces pombe Mediator in which conserved Mediator subunits are individually resolved. The essential Med14 subunit works as a central backbone that connects the Mediator head, middle and tail modules. Comparison with a 7.8 Å resolution cryo-electron microscopy map of a Mediator-RNA polymerase II holoenzyme reveals that changes in the structure of Med14 facilitate a large-scale Mediator rearrangement that is essential for holoenzyme formation. Our study suggests that access to different conformations and crosstalk between structural elements are essential for the Mediator regulation mechanism, and could explain the capacity of the complex to integrate multiple regulatory signals.
リンク	Nature / PubMed:28241144 / PubMed Central
手法	EM (単粒子)
解像度	4.4 - 7.8 Å
構造データ	8479 5u0p EMDB-8479: Cryo-EM map of the transcriptional Mediator PDB-5u0p: Cryo-EM structure of the transcriptional Mediator 手法: EM (単粒子) / 解像度: 4.4 Å 8480 5u0s EMDB-8480: Cryo-EM map of the Mediator-RNAPII complex PDB-5u0s: Cryo-EM structure of the Mediator-RNAPII complex 手法: EM (単粒子) / 解像度: 7.8 Å
由来	schizosaccharomyces pombe (分裂酵母) Fission yeast (分裂酵母)
キーワード	TRANSCRIPTION / transcriptional / Mediator / Head / Middle / Tail / Srb / RNA polymerase II / Med / TRANSCRIPTION/TRANSFERASE / RNA / Pol2 / activation / complex / TRANSCRIPTION-TRANSFERASE complex