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Structure paper

TitleStructures and ion transport mechanisms of plant high-affinity potassium transporters.
Journal, issue, pagesMol Plant, Vol. 17, Issue 3, Page 409-422, Year 2024
Publish dateMar 4, 2024
AuthorsJiangqin Wang / Yanping Luo / Fan Ye / Zhong Jie Ding / Shao Jian Zheng / Shuai Qiao / Yong Wang / Jiangtao Guo / Wei Yang / Nannan Su /
PubMed AbstractPlant high-affinity K transporters (HKTs) mediate Na and K uptake, maintain Na/K homeostasis, and therefore play crucial roles in plant salt tolerance. In this study, we present cryoelectron ...Plant high-affinity K transporters (HKTs) mediate Na and K uptake, maintain Na/K homeostasis, and therefore play crucial roles in plant salt tolerance. In this study, we present cryoelectron microscopy structures of HKTs from two classes, class I HKT1;1 from Arabidopsis thaliana (AtHKT1;1) and class II HKT2;1 from Triticum aestivum (TaHKT2;1), in both Na- and K-bound states at 2.6- to 3.0-Å resolutions. Both AtHKT1;1 and TaHKT2;1 function as homodimers. Each HKT subunit consists of four tandem domain units (D1-D4) with a repeated K-channel-like M-P-M topology. In each subunit, D1-D4 assemble into an ion conduction pore with a pseudo-four-fold symmetry. Although both TaHKT2;1 and AtHKT1;1 have only one putative Na ion bound in the selectivity filter with a similar coordination pattern, the two HKTs display different K binding modes in the filter. TaHKT2;1 has three K ions bound in the selectivity filter, but AtHKT1;1 has only two K ions bound in the filter, which has a narrowed external entrance due to the presence of a Ser residue in the first filter motif. These structures, along with computational, mutational, and electrophysiological analyses, enable us to pinpoint key residues that are critical for the ion selectivity of HKTs. The findings provide new insights into the ion selectivity and ion transport mechanisms of plant HKTs and improve our understanding about how HKTs mediate plant salt tolerance and enhance crop growth.
External linksMol Plant / PubMed:38335958
MethodsEM (single particle)
Resolution2.6 - 2.9 Å
Structure data

37376

8w9n

EMDB-37376, PDB-8w9n:
Structure of AtHKT1;1 in NaCl at 2.7 Angstroms resolution
Method: EM (single particle) / Resolution: 2.7 Å

37377

8w9o

EMDB-37377, PDB-8w9o:
structure of AtHKT1;1 in KCl at 2.8 Angstroms resolution
Method: EM (single particle) / Resolution: 2.8 Å

37381

8w9t

EMDB-37381, PDB-8w9t:
Structure of TaHKT2;1 in NaCl at 2.6 Angstroms resolution
Method: EM (single particle) / Resolution: 2.6 Å

37382

8w9v

EMDB-37382, PDB-8w9v:
structure of TaHKT2;1 in KCl at 2.9 Angstroms resolution
Method: EM (single particle) / Resolution: 2.9 Å

Chemicals

ChemComp-NA:
Unknown entry

ChemComp-K:
Unknown entry

ChemComp-HOH:
WATER

Source
  • arabidopsis thaliana (thale cress)
  • triticum aestivum (bread wheat)
KeywordsTRANSPORT PROTEIN / HKT / salt tolerance / ion selectivity

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