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-Structure paper
Title | Structures of the Insecticidal Toxin Complex Subunit XptA2 Highlight Roles for Flexible Domains. |
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Journal, issue, pages | Int J Mol Sci, Vol. 24, Issue 17, Year 2023 |
Publish date | Aug 25, 2023 |
Authors | Cole L Martin / David W Chester / Christopher D Radka / Lurong Pan / Zhengrong Yang / Rachel C Hart / Elad M Binshtein / Zhao Wang / Lisa Nagy / Lawrence J DeLucas / Stephen G Aller / |
PubMed Abstract | The Toxin Complex (Tc) superfamily consists of toxin translocases that contribute to the targeting, delivery, and cytotoxicity of certain pathogenic Gram-negative bacteria. Membrane receptor ...The Toxin Complex (Tc) superfamily consists of toxin translocases that contribute to the targeting, delivery, and cytotoxicity of certain pathogenic Gram-negative bacteria. Membrane receptor targeting is driven by the A-subunit (TcA), which comprises IgG-like receptor binding domains (RBDs) at the surface. To better understand XptA2, an insect specific TcA secreted by the symbiont from the intestine of entomopathogenic nematodes, we determined structures by X-ray crystallography and cryo-EM. Contrary to a previous report, XptA2 is pentameric. RBD-B exhibits an indentation from crystal packing that indicates loose association with the shell and a hotspot for possible receptor binding or a trigger for conformational dynamics. A two-fragment XptA2 lacking an intact linker achieved the folded pre-pore state like wild type (wt), revealing no requirement of the linker for protein folding. The linker is disordered in all structures, and we propose it plays a role in dynamics downstream of the initial pre-pore state. |
External links | Int J Mol Sci / PubMed:37686027 / PubMed Central |
Methods | EM (single particle) / X-ray diffraction |
Resolution | 3.1 Å |
Structure data | EMDB-41503, PDB-8tqe: PDB-8tv0: |
Source |
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Keywords | TOXIN / TcA / Insecticide / Translocase |