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Structure paper

TitleStructural and functional analysis of the minimal orthomyxovirus-like polymerase of Tilapia Lake Virus from the highly diverged Amnoonviridae family.
Journal, issue, pagesNat Commun, Vol. 14, Issue 1, Page 8145, Year 2023
Publish dateDec 9, 2023
AuthorsBenoit Arragain / Martin Pelosse / Albert Thompson / Stephen Cusack /
PubMed AbstractTilapia Lake Virus (TiLV), a recently discovered pathogen of tilapia fish, belongs to the Amnoonviridae family from the Articulavirales order. Its ten genome segments have characteristic conserved ...Tilapia Lake Virus (TiLV), a recently discovered pathogen of tilapia fish, belongs to the Amnoonviridae family from the Articulavirales order. Its ten genome segments have characteristic conserved ends and encode proteins with no known homologues, apart from the segment 1, which encodes an orthomyxo-like RNA-dependent-RNA polymerase core subunit. Here we show that segments 1-3 encode respectively the PB1, PB2 and PA-like subunits of an active heterotrimeric polymerase that maintains all domains found in the distantly related influenza polymerase, despite an unprecedented overall size reduction of 40%. Multiple high-resolution cryo-EM structures of TiLV polymerase in pre-initiation, initiation and active elongation states, show how it binds the vRNA and cRNA promoters and performs RNA synthesis, with both transcriptase and replicase configurations being characterised. However, the highly truncated endonuclease-like domain appears inactive and the putative cap-binding domain is autoinhibited, emphasising that many functional aspects of TiLV polymerase remain to be elucidated.
External linksNat Commun / PubMed:38066000 / PubMed Central
MethodsEM (single particle)
Resolution2.4 - 3.18 Å
Structure data

EMDB-17857, PDB-8psn:
Tilapia Lake Virus polymerase in vRNA initiation state (transcriptase conformation)
Method: EM (single particle) / Resolution: 2.73 Å

EMDB-17858, PDB-8pso:
Tilapia Lake Virus polymerase in vRNA initiation state (core only)
Method: EM (single particle) / Resolution: 2.4 Å

EMDB-17860, PDB-8psq:
Tilapia Lake Virus polymerase in cRNA pre-initiation state mode A (core only)
Method: EM (single particle) / Resolution: 2.65 Å

EMDB-17861, PDB-8pss:
Tilapia Lake Virus polymerase in cRNA pre-initiation state mode B (core-endo only)
Method: EM (single particle) / Resolution: 2.83 Å

EMDB-17862, PDB-8psu:
Tilapia Lake Virus polymerase in vRNA pre-initiation state mode A (core only)
Method: EM (single particle) / Resolution: 3.18 Å

EMDB-17864, PDB-8psx:
Tilapia Lake Virus polymerase in vRNA elongation state (transcriptase conformation)
Method: EM (single particle) / Resolution: 2.96 Å

EMDB-17865, PDB-8psz:
Tilapia Lake Virus polymerase in vRNA elongation state with additional mode B promoter (transcriptase conformation)
Method: EM (single particle) / Resolution: 2.42 Å

EMDB-17866, PDB-8pt2:
Tilapia Lake Virus polymerase in vRNA pre-initiation state mode B (transcriptase conformation)
Method: EM (single particle) / Resolution: 2.59 Å

EMDB-17868, PDB-8pt6:
Tilapia Lake Virus polymerase in vRNA initiation state (replicase conformation)
Method: EM (single particle) / Resolution: 3.03 Å

EMDB-17869, PDB-8pt7:
Tilapia Lake Virus polymerase in cRNA pre-initiation state mode A (core-endo only)
Method: EM (single particle) / Resolution: 2.8 Å

EMDB-17871, PDB-8pth:
Tilapia Lake Virus polymerase in vRNA pre-initiation state mode B (open core | partial replicase conformation)
Method: EM (single particle) / Resolution: 2.73 Å

EMDB-17872, PDB-8ptj:
Tilapia Lake Virus polymerase in vRNA pre-initiation state mode B (close core | partial replicase conformation)
Method: EM (single particle) / Resolution: 2.86 Å

EMDB-18772, PDB-8qz8:
Tilapia Lake Virus polymerase in vRNA pre-termination state (transcriptase conformation)
Method: EM (single particle) / Resolution: 3.13 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-A0I:
[(2~{R},3~{S},4~{R},5~{R})-5-(4-azanyl-2-oxidanylidene-pyrimidin-1-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-~{N}-[oxidanyl(phosphonooxy)phosphoryl]phosphonamidic acid

ChemComp-G2P:
PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / GMP-CPP, energy-carrying molecule analogue*YM

Source
  • tilapia lake virus
  • synthetic construct (others)
KeywordsVIRAL PROTEIN / Viral polymerase

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