Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structural and functional analysis of the minimal orthomyxovirus-like polymerase of Tilapia Lake Virus from the highly diverged Amnoonviridae family.
Journal, issue, pages	Nat Commun, Vol. 14, Issue 1, Page 8145, Year 2023
Publish date	Dec 9, 2023
Authors	Benoit Arragain / Martin Pelosse / Albert Thompson / Stephen Cusack /
PubMed Abstract	Tilapia Lake Virus (TiLV), a recently discovered pathogen of tilapia fish, belongs to the Amnoonviridae family from the Articulavirales order. Its ten genome segments have characteristic conserved ...Tilapia Lake Virus (TiLV), a recently discovered pathogen of tilapia fish, belongs to the Amnoonviridae family from the Articulavirales order. Its ten genome segments have characteristic conserved ends and encode proteins with no known homologues, apart from the segment 1, which encodes an orthomyxo-like RNA-dependent-RNA polymerase core subunit. Here we show that segments 1-3 encode respectively the PB1, PB2 and PA-like subunits of an active heterotrimeric polymerase that maintains all domains found in the distantly related influenza polymerase, despite an unprecedented overall size reduction of 40%. Multiple high-resolution cryo-EM structures of TiLV polymerase in pre-initiation, initiation and active elongation states, show how it binds the vRNA and cRNA promoters and performs RNA synthesis, with both transcriptase and replicase configurations being characterised. However, the highly truncated endonuclease-like domain appears inactive and the putative cap-binding domain is autoinhibited, emphasising that many functional aspects of TiLV polymerase remain to be elucidated.
External links	Nat Commun / PubMed:38066000 / PubMed Central
Methods	EM (single particle)
Resolution	2.4 - 3.18 Å
Structure data	17857 8psn EMDB-17857, PDB-8psn: Tilapia Lake Virus polymerase in vRNA initiation state (transcriptase conformation) Method: EM (single particle) / Resolution: 2.73 Å 17858 8pso EMDB-17858, PDB-8pso: Tilapia Lake Virus polymerase in vRNA initiation state (core only) Method: EM (single particle) / Resolution: 2.4 Å 17860 8psq EMDB-17860, PDB-8psq: Tilapia Lake Virus polymerase in cRNA pre-initiation state mode A (core only) Method: EM (single particle) / Resolution: 2.65 Å 17861 8pss EMDB-17861, PDB-8pss: Tilapia Lake Virus polymerase in cRNA pre-initiation state mode B (core-endo only) Method: EM (single particle) / Resolution: 2.83 Å 17862 8psu EMDB-17862, PDB-8psu: Tilapia Lake Virus polymerase in vRNA pre-initiation state mode A (core only) Method: EM (single particle) / Resolution: 3.18 Å 17864 8psx EMDB-17864, PDB-8psx: Tilapia Lake Virus polymerase in vRNA elongation state (transcriptase conformation) Method: EM (single particle) / Resolution: 2.96 Å 17865 8psz EMDB-17865, PDB-8psz: Tilapia Lake Virus polymerase in vRNA elongation state with additional mode B promoter (transcriptase conformation) Method: EM (single particle) / Resolution: 2.42 Å 17866 8pt2 EMDB-17866, PDB-8pt2: Tilapia Lake Virus polymerase in vRNA pre-initiation state mode B (transcriptase conformation) Method: EM (single particle) / Resolution: 2.59 Å 17868 8pt6 EMDB-17868, PDB-8pt6: Tilapia Lake Virus polymerase in vRNA initiation state (replicase conformation) Method: EM (single particle) / Resolution: 3.03 Å 17869 8pt7 EMDB-17869, PDB-8pt7: Tilapia Lake Virus polymerase in cRNA pre-initiation state mode A (core-endo only) Method: EM (single particle) / Resolution: 2.8 Å 17871 8pth EMDB-17871, PDB-8pth: Tilapia Lake Virus polymerase in vRNA pre-initiation state mode B (open core \| partial replicase conformation) Method: EM (single particle) / Resolution: 2.73 Å 17872 8ptj EMDB-17872, PDB-8ptj: Tilapia Lake Virus polymerase in vRNA pre-initiation state mode B (close core \| partial replicase conformation) Method: EM (single particle) / Resolution: 2.86 Å 18772 8qz8 EMDB-18772, PDB-8qz8: Tilapia Lake Virus polymerase in vRNA pre-termination state (transcriptase conformation) Method: EM (single particle) / Resolution: 3.13 Å
Chemicals	ChemComp-ZN: Unknown entry ChemComp-MG: Unknown entry ChemComp-HOH: WATER ChemComp-A0I: [(2~{R},3~{S},4~{R},5~{R})-5-(4-azanyl-2-oxidanylidene-pyrimidin-1-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-~{N}-[oxidanyl(phosphonooxy)phosphoryl]phosphonamidic acid ChemComp-G2P: PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER / GMP-CPP, energy-carrying molecule analogue*YM
Source	tilapia lake virus synthetic construct (others)
Keywords	VIRAL PROTEIN / Viral polymerase