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TitleVCP/p97-associated proteins are binders and debranching enzymes of K48-K63-branched ubiquitin chains.
Journal, issue, pagesNat. Struct. Mol. Biol., Year 2024
Publish dateJan 26, 2021 (structure data deposition date)
AuthorsLange, S.M. / McFarland, M.R. / Lamoliatte, F. / Carroll, T. / Krshnan, L. / Perez-Rafols, A. / Kwasna, D. / Shen, L. / Wallace, I. / Cole, I. ...Lange, S.M. / McFarland, M.R. / Lamoliatte, F. / Carroll, T. / Krshnan, L. / Perez-Rafols, A. / Kwasna, D. / Shen, L. / Wallace, I. / Cole, I. / Armstrong, L.A. / Knebel, A. / Johnson, C. / De Cesare, V. / Kulathu, Y.
External linksNat. Struct. Mol. Biol. / PubMed:38977901
MethodsX-ray diffraction
Resolution1.55 - 2.19 Å
Structure data

PDB-7nbb:
Branched Lys48- and Lys63-linked tri-ubiquitin (K48-K63-Ub3) in complex with synthetic nanobody NbSL3
Method: X-RAY DIFFRACTION / Resolution: 1.55 Å

PDB-7npo:
Branched K48-K63-Ub3
Method: X-RAY DIFFRACTION / Resolution: 2.19 Å

PDB-8a67:
Branched Lys48- and Lys63-linked tri-ubiquitin (K48-K63-Ub3) in complex with matured synthetic nanobody NbSL3.3Q (3rd generation)
Method: X-RAY DIFFRACTION / Resolution: 1.86 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-GOL:
GLYCEROL

ChemComp-CL:
Unknown entry

ChemComp-NA:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-IPA:
ISOPROPYL ALCOHOL / alkaloid*YM

ChemComp-ZN:
Unknown entry

Source
  • homo sapiens (human)
  • synthetic construct (others)
KeywordsSIGNALING PROTEIN / Ubiquitin / Branched / Heterotypic / Nanobody / Synthetic nanobody / Branched ubiquitin chains / heterotypic ubiquitin chains / K48-K63-Triubiquitin / K48-K63-Ub3 / Branched Ubiquitin / complex

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