Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structural insights into histone binding and nucleosome assembly by chromatin assembly factor-1.
Journal, issue, pages	Science, Vol. 381, Issue 6660, Page eadd8673, Year 2023
Publish date	Aug 25, 2023
Authors	Chao-Pei Liu / Zhenyu Yu / Jun Xiong / Jie Hu / Aoqun Song / Dongbo Ding / Cong Yu / Na Yang / Mingzhu Wang / Juan Yu / Peini Hou / Kangning Zeng / Zhenyu Li / Zhuqiang Zhang / Xinzheng Zhang / Wei Li / Zhiguo Zhang / Bing Zhu / Guohong Li / Rui-Ming Xu /
PubMed Abstract	Chromatin inheritance entails de novo nucleosome assembly after DNA replication by chromatin assembly factor-1 (CAF-1). Yet direct knowledge about CAF-1's histone binding mode and nucleosome assembly ...Chromatin inheritance entails de novo nucleosome assembly after DNA replication by chromatin assembly factor-1 (CAF-1). Yet direct knowledge about CAF-1's histone binding mode and nucleosome assembly process is lacking. In this work, we report the crystal structure of human CAF-1 in the absence of histones and the cryo-electron microscopy structure of CAF-1 in complex with histones H3 and H4. One histone H3-H4 heterodimer is bound by one CAF-1 complex mainly through the p60 subunit and the acidic domain of the p150 subunit. We also observed a dimeric CAF-1-H3-H4 supercomplex in which two H3-H4 heterodimers are poised for tetramer assembly and discovered that CAF-1 facilitates right-handed DNA wrapping of H3-H4 tetramers. These findings signify the involvement of DNA in H3-H4 tetramer formation and suggest a right-handed nucleosome precursor in chromatin replication.
External links	Science / PubMed:37616371 / PubMed Central
Methods	EM (single particle) / X-ray diffraction
Resolution	3.42 - 6.6 Å
Structure data	33625 7y5u EMDB-33625, PDB-7y5u: Cryo-EM structure of the monomeric human CAF1LC-H3-H4 complex Method: EM (single particle) / Resolution: 3.8 Å 33626 7y5v EMDB-33626, PDB-7y5v: Cryo-EM structure of the dimeric human CAF1LC-H3-H4 complex Method: EM (single particle) / Resolution: 6.1 Å 33627 7y5w EMDB-33627, PDB-7y5w: Cryo-EM structure of the left-handed Di-tetrasome Method: EM (single particle) / Resolution: 3.5 Å 33630 7y60 EMDB-33630, PDB-7y60: Cryo-EM structure of human CAF1LC bound right-handed Di-tetrasome Method: EM (single particle) / Resolution: 3.8 Å 33631 7y61 EMDB-33631, PDB-7y61: Cryo-EM structure of the two CAF1LCs bound right-handed Di-tetrasome Method: EM (single particle) / Resolution: 5.6 Å 35660 8iqf EMDB-35660: Composite cryo-EM density map of the dimeric human CAF1-H3-H4 complex PDB-8iqf: Cryo-EM structure of the dimeric human CAF1-H3-H4 complex Method: EM (single particle) / Resolution: 4.6 Å 35661 8iqg EMDB-35661, PDB-8iqg: Cryo-EM structure of the monomeric human CAF1-H3-H4 complex Method: EM (single particle) / Resolution: 3.5 Å EMDB-35708: Cryo-EM consensus map of the dimeric human CAF1-H3-H4 complex Method: EM (single particle) / Resolution: 5.9 Å EMDB-35709: Local refinement cryo-EM map of protomer I of the dimeric human CAF1-H3-H4 complex Method: EM (single particle) / Resolution: 4.63 Å EMDB-35710: Local refinement cryo-EM map of protomer II of the dimeric human CAF1-H3-H4 complex Method: EM (single particle) / Resolution: 4.58 Å 36013 8j6s EMDB-36013, PDB-8j6s: Cryo-EM structure of the single CAF-1 bound right-handed Di-tetrasome Method: EM (single particle) / Resolution: 3.8 Å 36014 8j6t EMDB-36014, PDB-8j6t: Cryo-EM structure of the double CAF-1 bound right-handed Di-tetrasome Method: EM (single particle) / Resolution: 6.6 Å PDB-7y5k: Crystal structure of human CAF-1 core complex in spacegroup C2221 Method: X-RAY DIFFRACTION / Resolution: 3.48 Å PDB-7y5l: Crystal structure of human CAF-1 core complex in spacegroup C2 Method: X-RAY DIFFRACTION / Resolution: 3.42 Å PDB-7y5o: Crystal structure of human CAF-1 core complex in spacegroup P21 Method: X-RAY DIFFRACTION / Resolution: 3.57 Å
Chemicals	ChemComp-GOL: GLYCEROL ChemComp-HOH: WATER
Source	homo sapiens (human) synthetic construct (others)
Keywords	REPLICATION / Histone chaperone / Chromatin assembly factor / DNA BINDING PROTEIN/DNA / Left-handed / Di-tetrasome / DNA BINDING PROTEIN / DNA BINDING PROTEIN-DNA complex / REPLICATION/DNA / REPLICATION-DNA COMPLEX