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TitlePseudomonas aeruginosa SutA wedges RNAP lobe domain open to facilitate promoter DNA unwinding.
Journal, issue, pagesNat Commun, Vol. 13, Issue 1, Page 4204, Year 2022
Publish dateJul 20, 2022
AuthorsDingwei He / Linlin You / Xiaoxian Wu / Jing Shi / Aijia Wen / Zhi Yan / Wenhui Mu / Chengli Fang / Yu Feng / Yu Zhang /
PubMed AbstractPseudomonas aeruginosa (Pae) SutA adapts bacteria to hypoxia and nutrition-limited environment during chronic infection by increasing transcription activity of an RNA polymerase (RNAP) holoenzyme ...Pseudomonas aeruginosa (Pae) SutA adapts bacteria to hypoxia and nutrition-limited environment during chronic infection by increasing transcription activity of an RNA polymerase (RNAP) holoenzyme comprising the stress-responsive σ factor σ (RNAP-σ). SutA shows no homology to previously characterized RNAP-binding proteins. The structure and mode of action of SutA remain unclear. Here we determined cryo-EM structures of Pae RNAP-σ holoenzyme, Pae RNAP-σ holoenzyme complexed with SutA, and Pae RNAP-σ transcription initiation complex comprising SutA. The structures show SutA pinches RNAP-β protrusion and facilitates promoter unwinding by wedging RNAP-β lobe open. Our results demonstrate that SutA clears an energetic barrier to facilitate promoter unwinding of RNAP-σ holoenzyme.
External linksNat Commun / PubMed:35859063 / PubMed Central
MethodsEM (single particle)
Resolution3.13 - 5.8 Å
Structure data

31403

7f0r

EMDB-31403, PDB-7f0r:
Cryo-EM structure of Pseudomonas aeruginosa SutA transcription activation complex
Method: EM (single particle) / Resolution: 5.8 Å

31948

7vf9

EMDB-31948, PDB-7vf9:
Cryo-EM structure of Pseudomonas aeruginosa RNAP sigmaS holoenzyme complexes
Method: EM (single particle) / Resolution: 4.04 Å

33271

7xl3

EMDB-33271, PDB-7xl3:
Cryo-EM structure of Pseudomonas aeruginosa RNAP sigmaS holoenzyme complexes with transcription factor SutA (open lobe)
Method: EM (single particle) / Resolution: 3.13 Å

33272

7xl4

EMDB-33272, PDB-7xl4:
Cryo-EM structure of Pseudomonas aeruginosa RNAP sigmaS holoenzyme complexes with transcription factor SutA (closed lobe)
Method: EM (single particle) / Resolution: 3.86 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-MG:
Unknown entry

Source
  • pseudomonas aeruginosa pao1 (bacteria)
  • pseudomonas aeruginosa (strain atcc 15692 / dsm 22644 / cip 104116 / jcm 14847 / lmg 12228 / 1c / prs 101 / pao1) (bacteria)
  • synthetic construct (others)
KeywordsTRANSCRIPTION/DNA / transcription initiation / Pseudomonas aeruginosa / SutA / transcription activation / sigmaS / RNA polymerase / RNAP beta lobe / TRANSLATION / TRANSCRIPTION-DNA complex / TRANSCRIPTION / closed beta lobe / holoenzyme / open beta lobe

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