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TitleStructural insight into mitochondrial β-barrel outer membrane protein biogenesis.
Journal, issue, pagesNat Commun, Vol. 11, Issue 1, Page 3290, Year 2020
Publish dateJul 3, 2020
AuthorsKathryn A Diederichs / Xiaodan Ni / Sarah E Rollauer / Istvan Botos / Xiaofeng Tan / Martin S King / Edmund R S Kunji / Jiansen Jiang / Susan K Buchanan /
PubMed AbstractIn mitochondria, β-barrel outer membrane proteins mediate protein import, metabolite transport, lipid transport, and biogenesis. The Sorting and Assembly Machinery (SAM) complex consists of three ...In mitochondria, β-barrel outer membrane proteins mediate protein import, metabolite transport, lipid transport, and biogenesis. The Sorting and Assembly Machinery (SAM) complex consists of three proteins that assemble as a 1:1:1 complex to fold β-barrel proteins and insert them into the mitochondrial outer membrane. We report cryoEM structures of the SAM complex from Myceliophthora thermophila, which show that Sam50 forms a 16-stranded transmembrane β-barrel with a single polypeptide-transport-associated (POTRA) domain extending into the intermembrane space. Sam35 and Sam37 are located on the cytosolic side of the outer membrane, with Sam35 capping Sam50, and Sam37 interacting extensively with Sam35. Sam35 and Sam37 each adopt a GST-like fold, with no functional, structural, or sequence similarity to their bacterial counterparts. Structural analysis shows how the Sam50 β-barrel opens a lateral gate to accommodate its substrates.
External linksNat Commun / PubMed:32620929 / PubMed Central
MethodsEM (single particle)
Resolution3.0 - 3.9 Å
Structure data

21913

6wuh

EMDB-21913, PDB-6wuh:
Mitochondrial SAM complex in lipid nanodiscs
Method: EM (single particle) / Resolution: 3.4 Å

21914

6wuj

EMDB-21914, PDB-6wuj:
Mitochondrial SAM complex - monomer in detergent
Method: EM (single particle) / Resolution: 3.7 Å

21915

6wul

EMDB-21915, PDB-6wul:
Mitochondrial SAM complex - dimer 1 in detergent
Method: EM (single particle) / Resolution: 3.2 Å

21916

6wum

EMDB-21916, PDB-6wum:
Mitochondrial SAM complex - dimer 2 in detergent
Method: EM (single particle) / Resolution: 3.6 Å

21917

6wun

EMDB-21917, PDB-6wun:
Mitochondrial SAM complex - dimer 3 in detergent
Method: EM (single particle) / Resolution: 3.9 Å

21918

6wut

EMDB-21918, PDB-6wut:
Mitochondrial SAM complex - high resolution monomer in detergent
Method: EM (single particle) / Resolution: 3.0 Å

Source
  • thermothelomyces thermophilus (fungus)
KeywordsMEMBRANE PROTEIN / Mitochondrial SAM complex / Sam35 / Sam37 / Sam50. / Sam50

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