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Structure paper

TitleStructural basis of redox modulation on chloroplast ATP synthase.
Journal, issue, pagesCommun Biol, Vol. 3, Issue 1, Page 482, Year 2020
Publish dateSep 2, 2020
AuthorsJay-How Yang / Dewight Williams / Eaazhisai Kandiah / Petra Fromme / Po-Lin Chiu /
PubMed AbstractIn higher plants, chloroplast ATP synthase has a unique redox switch on its γ subunit that modulates enzyme activity to limit ATP hydrolysis at night. To understand the molecular details of the ...In higher plants, chloroplast ATP synthase has a unique redox switch on its γ subunit that modulates enzyme activity to limit ATP hydrolysis at night. To understand the molecular details of the redox modulation, we used single-particle cryo-EM to determine the structures of spinach chloroplast ATP synthase in both reduced and oxidized states. The disulfide linkage of the oxidized γ subunit introduces a torsional constraint to stabilize the two β hairpin structures. Once reduced, free cysteines alleviate this constraint, resulting in a concerted motion of the enzyme complex and a smooth transition between rotary states to facilitate the ATP synthesis. We added an uncompetitive inhibitor, tentoxin, in the reduced sample to limit the flexibility of the enzyme and obtained high-resolution details. Our cryo-EM structures provide mechanistic insight into the redox modulation of the energy regulation activity of chloroplast ATP synthase.
External linksCommun Biol / PubMed:32879423 / PubMed Central
MethodsEM (single particle)
Resolution3.05 - 7.9 Å
Structure data

21235

6vm1

EMDB-21235, PDB-6vm1:
Chloroplast ATP synthase (C3, CF1FO)
Method: EM (single particle) / Resolution: 7.9 Å

21238

6vm4

EMDB-21238, PDB-6vm4:
Chloroplast ATP synthase (C2, CF1FO)
Method: EM (single particle) / Resolution: 7.08 Å

21239

6vmb

EMDB-21239, PDB-6vmb:
Chloroplast ATP synthase (C1, CF1FO)
Method: EM (single particle) / Resolution: 5.23 Å

21240

6vmd

EMDB-21240, PDB-6vmd:
Chloroplast ATP synthase (C1, CF1)
Method: EM (single particle) / Resolution: 4.53 Å

21241

6vmg

EMDB-21241, PDB-6vmg:
Chloroplast ATP synthase (O3, CF1FO)
Method: EM (single particle) / Resolution: 6.46 Å

21262

6vof

EMDB-21262, PDB-6vof:
Chloroplast ATP synthase (O2, CF1FO)
Method: EM (single particle) / Resolution: 4.51 Å

21263

6vog

EMDB-21263, PDB-6vog:
Chloroplast ATP synthase (O2, CF1)
Method: EM (single particle) / Resolution: 4.35 Å

21264

6voh

EMDB-21264, PDB-6voh:
Chloroplast ATP synthase (O1, CF1FO)
Method: EM (single particle) / Resolution: 4.16 Å

21265

6voi

EMDB-21265, PDB-6voi:
Chloroplast ATP synthase (O1, CF1)
Method: EM (single particle) / Resolution: 4.03 Å

21266

6voj

EMDB-21266, PDB-6voj:
Chloroplast ATP synthase (R3, CF1FO)
Method: EM (single particle) / Resolution: 4.34 Å

21267

6vok

EMDB-21267, PDB-6vok:
Chloroplast ATP synthase (R3, CF1)
Method: EM (single particle) / Resolution: 3.85 Å

21268

6vol

EMDB-21268, PDB-6vol:
Chloroplast ATP synthase (R2, CF1FO)
Method: EM (single particle) / Resolution: 4.06 Å

21269

6vom

EMDB-21269, PDB-6vom:
Chloroplast ATP synthase (R2, CF1)
Method: EM (single particle) / Resolution: 3.6 Å

21270

6von

EMDB-21270, PDB-6von:
Chloroplast ATP synthase (R1, CF1FO)
Method: EM (single particle) / Resolution: 3.35 Å

21271

6voo

EMDB-21271, PDB-6voo:
Chloroplast ATP synthase (R1, CF1)
Method: EM (single particle) / Resolution: 3.05 Å

Chemicals

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-TTX:
TENTOXIN / toxin*YM

Source
  • spinacia oleracea (spinach)
  • Spinach (spinach)
KeywordsPHOTOSYNTHESIS/TRANSLOCASE / CF1FO / ATP synthase / PHOTOSYNTHESIS / PHOTOSYNTHESIS-TRANSLOCASE complex / TRANSLOCASE

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