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TitleCryo-EM structure of the rhodopsin-Gαi-βγ complex reveals binding of the rhodopsin C-terminal tail to the gβ subunit.
Journal, issue, pagesElife, Vol. 8, Year 2019
Publish dateJun 28, 2019
AuthorsChing-Ju Tsai / Jacopo Marino / Ricardo Adaixo / Filip Pamula / Jonas Muehle / Shoji Maeda / Tilman Flock / Nicholas Mi Taylor / Inayatulla Mohammed / Hugues Matile / Roger Jp Dawson / Xavier Deupi / Henning Stahlberg / Gebhard Schertler /
PubMed AbstractOne of the largest membrane protein families in eukaryotes are G protein-coupled receptors (GPCRs). GPCRs modulate cell physiology by activating diverse intracellular transducers, prominently ...One of the largest membrane protein families in eukaryotes are G protein-coupled receptors (GPCRs). GPCRs modulate cell physiology by activating diverse intracellular transducers, prominently heterotrimeric G proteins. The recent surge in structural data has expanded our understanding of GPCR-mediated signal transduction. However, many aspects, including the existence of transient interactions, remain elusive. We present the cryo-EM structure of the light-sensitive GPCR rhodopsin in complex with heterotrimeric Gi. Our density map reveals the receptor C-terminal tail bound to the Gβ subunit of the G protein, providing a structural foundation for the role of the C-terminal tail in GPCR signaling, and of Gβ as scaffold for recruiting Gα subunits and G protein-receptor kinases. By comparing available complexes, we found a small set of common anchoring points that are G protein-subtype specific. Taken together, our structure and analysis provide new structural basis for the molecular events of the GPCR signaling pathway.
External linksElife / PubMed:31251171 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution1.9 - 4.38 Å
Structure data

4598

6qno

EMDB-4598, PDB-6qno:
Rhodopsin-Gi protein complex
Method: EM (single particle) / Resolution: 4.38 Å

PDB-6qnk:
Antibody FAB fragment targeting Gi protein heterotrimer
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

Chemicals

ChemComp-PG4:
TETRAETHYLENE GLYCOL / precipitant*YM

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-PGE:
TRIETHYLENE GLYCOL

ChemComp-MLT:
D-MALATE

ChemComp-HOH:
WATER

ChemComp-RET:
RETINAL

Source
  • homo sapiens (human)
  • bos taurus (cattle)
  • mus musculus (house mouse)
KeywordsIMMUNE SYSTEM / Antibody FAB fragment / SIGNALING PROTEIN / GPCR and G protein complex

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