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Structure paper

TitleA simple and versatile microfluidic device for efficient biomacromolecule crystallization and structural analysis by serial crystallography.
Journal, issue, pagesIucrj, Vol. 6, Page 454-464, Year 2019
Publish dateJul 4, 2018 (structure data deposition date)
Authorsde Wijn, R. / Hennig, O. / Roche, J. / Engilberge, S. / Rollet, K. / Fernandez-Millan, P. / Brillet, K. / Betat, H. / Morl, M. / Roussel, A. ...de Wijn, R. / Hennig, O. / Roche, J. / Engilberge, S. / Rollet, K. / Fernandez-Millan, P. / Brillet, K. / Betat, H. / Morl, M. / Roussel, A. / Girard, E. / Mueller-Dieckmann, C. / Fox, G.C. / Olieric, V. / Gavira, J.A. / Lorber, B. / Sauter, C.
External linksIucrj / PubMed:31098026
MethodsX-ray diffraction
Resolution1.55 - 2.536 Å
Structure data

PDB-6gzp:
Llama nanobody PorM_02 structure determined at room temperature by in-situ diffraction in ChipX microfluidic device
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-6hw1:
ROOM TEMPERATURE STRUCTURE OF LIPASE FROM T. LANUGINOSA AT 2.5 A RESOLUTION IN CHIPX MICROFLUIDIC DEVICE
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-6ibp:
Structure of a psychrophilic CCA-adding enzyme at room temperature in ChipX microfluidic device
Method: X-RAY DIFFRACTION / Resolution: 2.536 Å

PDB-6ibq:
Structure of a nonameric RNA duplex at room temperature in ChipX microfluidic device
Method: X-RAY DIFFRACTION / Resolution: 1.55 Å

PDB-6q3t:
Structure of Protease1 from Pyrococcus horikoshii at room temperature in ChipX microfluidic device
Method: X-RAY DIFFRACTION / Resolution: 2.15 Å

PDB-6q52:
Structure of a psychrophilic CCA-adding enzyme in complex with CMPcPP at room temperature in ChipX microfluidic device
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

ChemComp-PO4:
PHOSPHATE ION

ChemComp-MG:
Unknown entry

ChemComp-SO4:
SULFATE ION

ChemComp-2TM:
5'-O-[(S)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]methyl}phosphoryl]cytidine

Source
  • lama glama (llama)
  • thermomyces lanuginosus (fungus)
  • planococcus halocryophilus (bacteria)
  • rattus (rat)
  • pyrococcus horikoshii (archaea)
KeywordsIMMUNE SYSTEM / Llama nanobody / Porphyromonas gingivalis / PorM / HYDROLASE / LIPASE / RNA BINDING PROTEIN / tRNA maturation / tRNA nucleotidyltransferase / ChipX / psychrophilic enzyme / RNA / RNA duplex / GoU pair / nucleation / Xo4 / Crystallophore / Room temperature / Counterdiffusion

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