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TitleStructure of the chromatin remodelling enzyme Chd1 bound to a ubiquitinylated nucleosome.
Journal, issue, pagesElife, Vol. 7, Year 2018
Publish dateAug 6, 2018
AuthorsRamasubramanian Sundaramoorthy / Amanda L Hughes / Hassane El-Mkami / David G Norman / Helder Ferreira / Tom Owen-Hughes /
PubMed AbstractATP-dependent chromatin remodelling proteins represent a diverse family of proteins that share ATPase domains that are adapted to regulate protein-DNA interactions. Here, we present structures of the ...ATP-dependent chromatin remodelling proteins represent a diverse family of proteins that share ATPase domains that are adapted to regulate protein-DNA interactions. Here, we present structures of the Chd1 protein engaged with nucleosomes in the presence of the transition state mimic ADP-beryllium fluoride. The path of DNA strands through the ATPase domains indicates the presence of contacts conserved with single strand translocases and additional contacts with both strands that are unique to Snf2 related proteins. The structure provides connectivity between rearrangement of ATPase lobes to a closed, nucleotide bound state and the sensing of linker DNA. Two turns of linker DNA are prised off the surface of the histone octamer as a result of Chd1 binding, and both the histone H3 tail and ubiquitin conjugated to lysine 120 are re-orientated towards the unravelled DNA. This indicates how changes to nucleosome structure can alter the way in which histone epitopes are presented.
External linksElife / PubMed:30079888 / PubMed Central
MethodsEM (single particle)
Resolution4.5 - 10.0 Å
Structure data

4318

6ftx

EMDB-4318, PDB-6ftx:
Structure of the chromatin remodelling enzyme Chd1 bound to a ubiquitinylated nucleosome
Method: EM (single particle) / Resolution: 4.5 Å

4336

6g0l

EMDB-4336, PDB-6g0l:
Structure of two molecules of the chromatin remodelling enzyme Chd1 bound to a nucleosome
Method: EM (single particle) / Resolution: 10.0 Å

Chemicals

ChemComp-BEF:
BERYLLIUM TRIFLUORIDE ION

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

Source
  • xenopus laevis (African clawed frog)
  • synthetic construct (others)
  • saccharomyces cerevisiae (brewer's yeast)
  • homo sapiens (human)
  • petromyzon marinus (sea lamprey)
  • xenopus tropicalis (tropical clawed frog)
  • saccharomyces cerevisiae s288c (yeast)
KeywordsMOTOR PROTEIN / Chromatin remodellers

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