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Title | The cryo-EM Structure of a Novel 40S Kinetoplastid-Specific Ribosomal Protein. |
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Journal, issue, pages | Structure, Vol. 25, Issue 12, Page 1785-11794.e3, Year 2017 |
Publish date | Dec 5, 2017 |
Authors | Jailson Brito Querido / Eder Mancera-Martínez / Quentin Vicens / Anthony Bochler / Johana Chicher / Angelita Simonetti / Yaser Hashem / |
PubMed Abstract | Kinetoplastids are potentially lethal protozoan pathogens affecting more than 20 million people worldwide. There is a critical need for more specific targets for the development of safer anti- ...Kinetoplastids are potentially lethal protozoan pathogens affecting more than 20 million people worldwide. There is a critical need for more specific targets for the development of safer anti-kinetoplastid therapeutic molecules that can replace the scarce and highly cytotoxic current drugs. The kinetoplastid ribosome represents a potential therapeutic target due to its relative structural divergence when compared with its human counterpart. However, several kinetoplastid-specific ribosomal features remain uncharacterized. Here, we present the near-atomic cryoelectron microscopy structure of a novel bona fide kinetoplastid-specific ribosomal (r-) protein (KSRP) bound to the ribosome. KSRP is an essential protein located at the solvent face of the 40S subunit, where it binds and stabilizes kinetoplastid-specific domains of rRNA, suggesting its role in ribosome integrity. KSRP also interacts with the r-protein eS6 at a region that is only conserved in kinetoplastids. The kinetoplastid-specific ribosomal environment of KSRP provides a promising target for the design of safer anti-kinetoplastidian drugs. |
External links | Structure / PubMed:29107485 |
Methods | EM (single particle) |
Resolution | 2.9 - 4.4 Å |
Structure data | EMDB-3844: 80S from Trypanosoma cruzi EMDB-3845: PDB-5osg: |
Source |
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Keywords | RIBOSOME / Kinetoplastids / cryo-EM / KSRP |