Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structure of a yeast spliceosome at 3.6-angstrom resolution.
Journal, issue, pages	Science, Vol. 349, Issue 6253, Page 1182-1191, Year 2015
Publish date	Sep 11, 2015
Authors	Chuangye Yan / Jing Hang / Ruixue Wan / Min Huang / Catherine C L Wong / Yigong Shi /
PubMed Abstract	Splicing of precursor messenger RNA (pre-mRNA) in yeast is executed by the spliceosome, which consists of five small nuclear ribonucleoproteins (snRNPs), NTC (nineteen complex), NTC-related proteins ...Splicing of precursor messenger RNA (pre-mRNA) in yeast is executed by the spliceosome, which consists of five small nuclear ribonucleoproteins (snRNPs), NTC (nineteen complex), NTC-related proteins (NTR), and a number of associated enzymes and cofactors. Here, we report the three-dimensional structure of a Schizosaccharomyces pombe spliceosome at 3.6-angstrom resolution, revealed by means of single-particle cryogenic electron microscopy. This spliceosome contains U2 and U5 snRNPs, NTC, NTR, U6 small nuclear RNA, and an RNA intron lariat. The atomic model includes 10,574 amino acids from 37 proteins and four RNA molecules, with a combined molecular mass of approximately 1.3 megadaltons. Spp42 (Prp8 in Saccharomyces cerevisiae), the key protein component of the U5 snRNP, forms a central scaffold and anchors the catalytic center. Both the morphology and the placement of protein components appear to have evolved to facilitate the dynamic process of pre-mRNA splicing. Our near-atomic-resolution structure of a central spliceosome provides a molecular framework for mechanistic understanding of pre-mRNA splicing.
External links	Science / PubMed:26292707
Methods	EM (single particle)
Resolution	3.3 - 6.89 Å
Structure data	6413 3jb9 EMDB-6413, PDB-3jb9: Cryo-EM structure of the yeast spliceosome at 3.6 angstrom resolution Method: EM (single particle) / Resolution: 3.6 Å 6414 3jb9 EMDB-6414: Local map for Spp42 region of the yeast spliceosome at 3.42 angstrom resolution PDB-3jb9: Cryo-EM structure of the yeast spliceosome at 3.6 angstrom resolution Method: EM (single particle) / Resolution: 3.42 Å 6415 3jb9 EMDB-6415: Local map for reduced Spp42 region of the yeast spliceosome at 3.52 angstrom resolution PDB-3jb9: Cryo-EM structure of the yeast spliceosome at 3.6 angstrom resolution Method: EM (single particle) / Resolution: 3.52 Å 6416 3jb9 EMDB-6416: Local map for Cwf10/Sm region of the yeast spliceosome at 3.30 angstrom resolution PDB-3jb9: Cryo-EM structure of the yeast spliceosome at 3.6 angstrom resolution Method: EM (single particle) / Resolution: 3.3 Å 6417 3jb9 EMDB-6417: Local map for reduced Cwf10/Sm region of the yeast spliceosome at 3.44 angstrom resolution PDB-3jb9: Cryo-EM structure of the yeast spliceosome at 3.6 angstrom resolution Method: EM (single particle) / Resolution: 3.44 Å 6418 3jb9 EMDB-6418: Local map for cetral region of the yeast spliceosome at 3.57 angstrom resolution PDB-3jb9: Cryo-EM structure of the yeast spliceosome at 3.6 angstrom resolution Method: EM (single particle) / Resolution: 3.57 Å 6419 3jb9 EMDB-6419: Local map for reduced central region of the yeast spliceosome at 3.96 angstrom resolution PDB-3jb9: Cryo-EM structure of the yeast spliceosome at 3.6 angstrom resolution Method: EM (single particle) / Resolution: 3.96 Å 6420 3jb9 EMDB-6420: Local map for Cwf17/Cwf8 region of the yeast spliceosome at 4.28 angstrom resolution PDB-3jb9: Cryo-EM structure of the yeast spliceosome at 3.6 angstrom resolution Method: EM (single particle) / Resolution: 4.28 Å 6421 3jb9 EMDB-6421: Local map for Cwf11/U2 region of the yeast spliceosome at 6.89 angstrom resolution PDB-3jb9: Cryo-EM structure of the yeast spliceosome at 3.6 angstrom resolution Method: EM (single particle) / Resolution: 6.89 Å
Chemicals	ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying moleculeYM ChemComp-MG: Unknown entry ChemComp-ZN: Unknown entry ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying moleculeYM
Source	schizosaccharomyces pombe (fission yeast) schizosaccharomyces pombe 972h- (yeast)
Keywords	RNA BINDING PROTEIN/RNA / Spliceosome / U2/U5/U6 / Lariat / RNA BINDING PROTEIN-RNA complex