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-Structure paper
Title | Structural and mechanistic insights into a lysosomal membrane enzyme HGSNAT involved in Sanfilippo syndrome. |
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Journal, issue, pages | Nat Commun, Vol. 15, Issue 1, Page 5388, Year 2024 |
Publish date | Jun 25, 2024 |
Authors | Boyang Zhao / Zhongzheng Cao / Yi Zheng / Phuong Nguyen / Alisa Bowen / Robert H Edwards / Robert M Stroud / Yi Zhou / Menno Van Lookeren Campagne / Fei Li / |
PubMed Abstract | Heparan sulfate (HS) is degraded in lysosome by a series of glycosidases. Before the glycosidases can act, the terminal glucosamine of HS must be acetylated by the integral lysosomal membrane enzyme ...Heparan sulfate (HS) is degraded in lysosome by a series of glycosidases. Before the glycosidases can act, the terminal glucosamine of HS must be acetylated by the integral lysosomal membrane enzyme heparan-α-glucosaminide N-acetyltransferase (HGSNAT). Mutations of HGSNAT cause HS accumulation and consequently mucopolysaccharidosis IIIC, a devastating lysosomal storage disease characterized by progressive neurological deterioration and early death where no treatment is available. HGSNAT catalyzes a unique transmembrane acetylation reaction where the acetyl group of cytosolic acetyl-CoA is transported across the lysosomal membrane and attached to HS in one reaction. However, the reaction mechanism remains elusive. Here we report six cryo-EM structures of HGSNAT along the reaction pathway. These structures reveal a dimer arrangement and a unique structural fold, which enables the elucidation of the reaction mechanism. We find that a central pore within each monomer traverses the membrane and controls access of cytosolic acetyl-CoA to the active site at its luminal mouth where glucosamine binds. A histidine-aspartic acid catalytic dyad catalyzes the transfer reaction via a ternary complex mechanism. Furthermore, the structures allow the mapping of disease-causing variants and reveal their potential impact on the function, thus creating a framework to guide structure-based drug discovery efforts. |
External links | Nat Commun / PubMed:38918376 / PubMed Central |
Methods | EM (single particle) |
Resolution | 2.92 - 3.61 Å |
Structure data | EMDB-43319, PDB-8vkj: EMDB-43338, PDB-8vlg: EMDB-43339, PDB-8vli: EMDB-43344, PDB-8vlu: EMDB-43345, PDB-8vlv: EMDB-43348, PDB-8vly: |
Chemicals | ChemComp-NAG: ChemComp-ACO: PDB-1acy: ChemComp-COA: PDB-1ac0: |
Source |
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Keywords | MEMBRANE PROTEIN / Lysosome / Transmembrane acetyltransferase / Acetyl-CoA / Heperan sulfate / TRANSFERASE/SUBSTRATE / substrate / Transferase-substrate analog complex / TRANSFERASE-SUBSTRATE complex / TRANSFERASE/INHIBITOR / CoA / product / TRANSFERASE-INHIBITOR complex / TRANSFERASE |