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TitleStructure of the SARS-CoV-2 Frameshift Stimulatory Element with an Upstream Multibranch Loop.
Journal, issue, pagesBiochemistry, Vol. 63, Issue 10, Page 1287-1296, Year 2024
Publish dateMay 21, 2024
AuthorsJake M Peterson / Scott T Becker / Collin A O'Leary / Puneet Juneja / Yang Yang / Walter N Moss /
PubMed AbstractThe severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) frameshift stimulatory element (FSE) is necessary for programmed -1 ribosomal frameshifting (-1 PRF) and optimized viral efficacy. The ...The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) frameshift stimulatory element (FSE) is necessary for programmed -1 ribosomal frameshifting (-1 PRF) and optimized viral efficacy. The FSE has an abundance of context-dependent alternate conformations, but two of the structures most crucial to -1 PRF are an attenuator hairpin and a three-stem H-type pseudoknot structure. A crystal structure of the pseudoknot alone features three RNA stems in a helically stacked linear structure, whereas a 6.9 Å cryo-EM structure including the upstream heptameric slippery site resulted in a bend between two stems. Our previous research alluded to an extended upstream multibranch loop that includes both the attenuator hairpin and the slippery site-a conformation not previously modeled. We aim to provide further context to the SARS-CoV-2 FSE via computational and medium resolution cryo-EM approaches, by presenting a 6.1 Å cryo-EM structure featuring a linear pseudoknot structure and a dynamic upstream multibranch loop.
External linksBiochemistry / PubMed:38727003
MethodsEM (single particle)
Resolution6.1 Å
Structure data

43137

8vci

EMDB-43137, PDB-8vci:
SARS-CoV-2 Frameshift Stimulatory Element with Upstream Multibranch Loop
Method: EM (single particle) / Resolution: 6.1 Å

Source
  • severe acute respiratory syndrome coronavirus 2
KeywordsRNA / Coronavirus / SARS-CoV-2 / Programmed -1 Ribosomal Frameshifting / -1 PRF / Frameshift Stimulatory Element / FSE / Attenuator Hairpin

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