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TitleStructures of the Mycobacterium tuberculosis efflux pump EfpA reveal the mechanisms of transport and inhibition.
Journal, issue, pagesNat Commun, Vol. 15, Issue 1, Page 7710, Year 2024
Publish dateSep 4, 2024
AuthorsShuhui Wang / Kun Wang / Kangkang Song / Zon Weng Lai / Pengfei Li / Dongying Li / Yajie Sun / Ye Mei / Chen Xu / Maofu Liao /
PubMed AbstractAs the first identified multidrug efflux pump in Mycobacterium tuberculosis (Mtb), EfpA is an essential protein and promising drug target. However, the functional and inhibitory mechanisms of EfpA ...As the first identified multidrug efflux pump in Mycobacterium tuberculosis (Mtb), EfpA is an essential protein and promising drug target. However, the functional and inhibitory mechanisms of EfpA are poorly understood. Here we report cryo-EM structures of EfpA in outward-open conformation, either bound to three endogenous lipids or the inhibitor BRD-8000.3. Three lipids inside EfpA span from the inner leaflet to the outer leaflet of the membrane. BRD-8000.3 occupies one lipid site at the level of inner membrane leaflet, competitively inhibiting lipid binding. EfpA resembles the related lysophospholipid transporter MFSD2A in both overall structure and lipid binding sites and may function as a lipid flippase. Combining AlphaFold-predicted EfpA structure, which is inward-open, we propose a complete conformational transition cycle for EfpA. Together, our results provide a structural and mechanistic foundation to comprehend EfpA function and develop EfpA-targeting anti-TB drugs.
External linksNat Commun / PubMed:39231991 / PubMed Central
MethodsEM (single particle)
Resolution3.04 - 3.68 Å
Structure data

37641

8wm5

EMDB-37641, PDB-8wm5:
multidrug transporter EfpA from Mycobacterium tuberculosis bound with lipids
Method: EM (single particle) / Resolution: 3.04 Å

42204

8ufd

EMDB-42204, PDB-8ufd:
Multidrug efflux pump MtEfpA bound with inhibitor BRD8000.3
Method: EM (single particle) / Resolution: 3.26 Å

42205

8ufe

EMDB-42205, PDB-8ufe:
Multidrug efflux pump EfpA from mycobacterium smegmatis
Method: EM (single particle) / Resolution: 3.68 Å

Chemicals

ChemComp-PTY:
PHOSPHATIDYLETHANOLAMINE / phospholipid*YM


ChemComp, No image

ChemComp-WJI:
Unknown entry

ChemComp-CDL:
CARDIOLIPIN / phospholipid*YM

Source
  • mycobacterium tuberculosis h37rv (bacteria)
  • mycobacterium tuberculosis (bacteria)
  • mycolicibacterium smegmatis (bacteria)
KeywordsTRANSPORT PROTEIN / multidrug efflux pump / EfpA / mycobacterium / BRD8000.3 / Mycobacterium smegmatis / multidrug transporter / lipid

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