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-Structure paper
Title | Structural Basis of RNA Polymerase I Transcription Initiation. |
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Journal, issue, pages | Cell, Vol. 169, Issue 1, Page 120-131.e22, Year 2017 |
Publish date | Mar 23, 2017 |
Authors | Christoph Engel / Tobias Gubbey / Simon Neyer / Sarah Sainsbury / Christiane Oberthuer / Carlo Baejen / Carrie Bernecky / Patrick Cramer / |
PubMed Abstract | Transcription initiation at the ribosomal RNA promoter requires RNA polymerase (Pol) I and the initiation factors Rrn3 and core factor (CF). Here, we combine X-ray crystallography and cryo-electron ...Transcription initiation at the ribosomal RNA promoter requires RNA polymerase (Pol) I and the initiation factors Rrn3 and core factor (CF). Here, we combine X-ray crystallography and cryo-electron microscopy (cryo-EM) to obtain a molecular model for basal Pol I initiation. The three-subunit CF binds upstream promoter DNA, docks to the Pol I-Rrn3 complex, and loads DNA into the expanded active center cleft of the polymerase. DNA unwinding between the Pol I protrusion and clamp domains enables cleft contraction, resulting in an active Pol I conformation and RNA synthesis. Comparison with the Pol II system suggests that promoter specificity relies on a distinct "bendability" and "meltability" of the promoter sequence that enables contacts between initiation factors, DNA, and polymerase. |
External links | Cell / PubMed:28340337 |
Methods | EM (single particle) / X-ray diffraction |
Resolution | 3.2 - 7.7 Å |
Structure data | EMDB-3590, PDB-5n5y: EMDB-3591, PDB-5n5z: EMDB-3592, PDB-5n60: EMDB-3593, PDB-5n61: EMDB-3594: RNA polymerase I initially transcribing complex with visible tandem Winged Helix domain PDB-5o7x: |
Chemicals | ChemComp-ZN: ChemComp-SO4: ChemComp-MG: |
Source |
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Keywords | TRANSCRIPTION / RNA polymerase I initiation / TRANSFERASE / RNA polymerase I / initially transcribing complex / CORE FACTOR |